PMID- 12092814
OWN - NLM
STAT- MEDLINE
DCOM- 20021227
LR  - 20190910
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 66
IP  - 5
DP  - 2002 May
TI  - Replication timing properties within the mouse distal chromosome 7 imprinting 
      cluster.
PG  - 1046-51
AB  - Genomic imprinting is characterized by allele-specific expression of genes within 
      chromosomal domains. Here we show, using fluorescence in situ hybridization 
      (FISH) analysis, that the large chromosomal domain of the mouse distal chromosome 
      7 imprinting cluster, approximately 1 Mb in length between p57Kip2 and H19 genes, 
      replicates asynchronously between the two alleles during S-phase. At the 
      telomeric side of this domain, we found a transition from asynchronous 
      replication at the imprinted p57Kip2 gene to synchronous replication at the Nap2 
      gene. Two-color FISH suggested that the paternal allele of this whole domain 
      replicates earlier than its maternal allele. Treatment of the cells with a 
      histone deacetylase inhibitor abolished this allele-specific feature accompanied 
      with accelerated replication of the later-replicating allele at a domain level. 
      Allele-specific asynchronous replication was observed even in ES cells. These 
      results suggest that this imprinting cluster consists of a large replication 
      domain which is already found at the early stage in development.
FAU - Kagotani, Kazuhiro
AU  - Kagotani K
AD  - Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie 
      University, Tsu, Japan.
FAU - Takebayashi, Shin-ichiro
AU  - Takebayashi S
FAU - Kohda, Atsushi
AU  - Kohda A
FAU - Taguchi, Hiroshi
AU  - Taguchi H
FAU - Paulsen, Martina
AU  - Paulsen M
FAU - Walter, Jorn
AU  - Walter J
FAU - Reik, Wolf
AU  - Reik W
FAU - Okumura, Katsuzumi
AU  - Okumura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Histone Deacetylase Inhibitors)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - *Cell Cycle/drug effects
MH  - *Chromosome Mapping
MH  - Enzyme Inhibitors/pharmacology
MH  - *Genomic Imprinting
MH  - Histone Deacetylase Inhibitors
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
EDAT- 2002/07/03 10:00
MHDA- 2002/12/28 04:00
CRDT- 2002/07/03 10:00
PHST- 2002/07/03 10:00 [pubmed]
PHST- 2002/12/28 04:00 [medline]
PHST- 2002/07/03 10:00 [entrez]
AID - 10.1271/bbb.66.1046 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2002 May;66(5):1046-51. doi: 10.1271/bbb.66.1046.