PMID- 12095550
OWN - NLM
STAT- MEDLINE
DCOM- 20020809
LR  - 20220317
IS  - 0360-3016 (Print)
IS  - 0360-3016 (Linking)
VI  - 53
IP  - 4
DP  - 2002 Jul 15
TI  - Expression of hypoxia-inducible factor-1alpha in cervical carcinomas: correlation 
      with tumor oxygenation.
PG  - 854-61
AB  - PURPOSE: To investigate the relations between hypoxia-inducible factor-1 (HIF-1), 
      tumor oxygenation, and clinical correlates in patients with locally advanced 
      carcinoma of the uterine cervix. METHODS AND MATERIALS: Biopsies from 42 patients 
      with invasive cervical carcinoma and previous polarographic O2 measurements were 
      assessed for the expression of HIF-1alpha using digitized microscopic imaging and 
      analysis. RESULTS: The HIF-1alpha expression levels ranged from <0.1% to 10.7% of 
      the total tumor area; the positive staining was localized exclusively to the 
      nuclei. Three distinct arrangement patterns of HIF-1alpha-positive cells in 
      relation to blood vessels were identified using spatial image mapping: (1) most 
      HIF-1alpha-positive cells were located within the typical oxygen diffusion 
      distance in tissue (< or =150 microm to the nearest blood vessel); (2) most 
      HIF-1alpha-positive cells were located in the vicinity (< or =60 microm) of the 
      blood vessels; and (3) no apparent spatial relationship was found between 
      HIF-1alpha-positive cells and blood vessels. A statistically significant 
      association was found between HIF-1alpha expression and tumor oxygenation 
      (Spearman correlation coefficient = 0.4, p <0.01), as determined with the 
      Eppendorf pO2 histograph. No correlation was found between the level of 
      HIF-1alpha expression and patient outcome, using disease-free survival as the end 
      point. CONCLUSION: Our results suggest that HIF-1alpha expression may represent a 
      useful biologic marker for hypoxia in uterine cervical cancer.
FAU - Haugland, Hans Kristian
AU  - Haugland HK
AD  - Department of Medical Biophysics, Ontario Cancer Institute/Princess Margaret 
      Hospital, Toronto, Ontario, Canada.
FAU - Vukovic, Vojislav
AU  - Vukovic V
FAU - Pintilie, Melania
AU  - Pintilie M
FAU - Fyles, Anthony W
AU  - Fyles AW
FAU - Milosevic, Michael
AU  - Milosevic M
FAU - Hill, Richard P
AU  - Hill RP
FAU - Hedley, David W
AU  - Hedley DW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Radiat Oncol Biol Phys
JT  - International journal of radiation oncology, biology, physics
JID - 7603616
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Transcription Factors)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Aged
MH  - Carcinoma/*metabolism
MH  - Disease-Free Survival
MH  - Female
MH  - Humans
MH  - Hypoxia
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Middle Aged
MH  - Oxygen/*metabolism
MH  - Time Factors
MH  - Transcription Factors/*metabolism
MH  - Uterine Cervical Neoplasms/*metabolism
EDAT- 2002/07/04 10:00
MHDA- 2002/08/10 10:01
CRDT- 2002/07/04 10:00
PHST- 2002/07/04 10:00 [pubmed]
PHST- 2002/08/10 10:01 [medline]
PHST- 2002/07/04 10:00 [entrez]
AID - S0360301602028158 [pii]
AID - 10.1016/s0360-3016(02)02815-8 [doi]
PST - ppublish
SO  - Int J Radiat Oncol Biol Phys. 2002 Jul 15;53(4):854-61. doi: 
      10.1016/s0360-3016(02)02815-8.