PMID- 12096201
OWN - NLM
STAT- MEDLINE
DCOM- 20020801
LR  - 20220321
IS  - 0040-6376 (Print)
IS  - 1468-3296 (Electronic)
IS  - 0040-6376 (Linking)
VI  - 57
IP  - 7
DP  - 2002 Jul
TI  - Increased levels of the chemokines GROalpha and MCP-1 in sputum samples from 
      patients with COPD.
PG  - 590-5
AB  - BACKGROUND: Patients with chronic obstructive pulmonary disease (COPD) have 
      increased numbers of neutrophils and macrophages in their lungs. Growth related 
      oncogene-alpha (GROalpha) attracts neutrophils, whereas monocyte chemoattractant 
      protein-1 (MCP-1) attracts monocytes that can differentiate into macrophages. The 
      aim of this study was to determine the concentration of GROalpha and MCP-1 in 
      bronchoalveolar lavage (BAL) fluid and sputum from non-smokers, healthy smokers 
      and patients with COPD, and to see if there was a correlation between the 
      concentrations of these chemokines, lung function, and numbers of inflammatory 
      cells. METHODS: BAL fluid and sputum from non-smokers (n=32), healthy smokers 
      (n=36), and patients with COPD (n=40) were analysed for the presence of GROalpha 
      and MCP-1 using ELISA. Cells counts were performed on the samples and 
      correlations between the concentrations of these chemokines, lung function, and 
      inflammatory cells observed. RESULTS: Median (SE) GROalpha and MCP-1 levels were 
      significantly increased in sputum from patients with COPD compared with 
      non-smokers and healthy smokers (GROalpha: 31 (11) v 2 (2) v 3 (0.8) ng/ml; 
      MCP-1: 0.8 (0.4) v 0.2 (0.1) v 0.1 (0.04) ng/ml, p<0.05), but not in BAL fluid. 
      There were significant negative correlations between both GROalpha and MCP-1 
      levels in sputum and forced expiratory volume in 1 second (FEV(1)) % predicted 
      (GROalpha: r=-0.5, p<0.001; MCP-1: r=-0.5, p<0.001), together with significant 
      positive correlations between GROalpha and MCP-1 and neutrophil numbers in sputum 
      (GROalpha: r=0.6, p<0.001; MCP-1: r=0.4, p<0.01). CONCLUSION: These results 
      suggest that GROalpha and MCP-1 are involved in the migration of inflammatory 
      cells, thus contributing to the inflammatory load associated with COPD.
FAU - Traves, S L
AU  - Traves SL
AD  - Department of Thoracic Medicine, National Heart and Lung Institute, Imperial 
      College, School of Medicine, Dovehouse Street, London SW3 6LY, UK.
FAU - Culpitt, S V
AU  - Culpitt SV
FAU - Russell, R E K
AU  - Russell RE
FAU - Barnes, P J
AU  - Barnes PJ
FAU - Donnelly, L E
AU  - Donnelly LE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Thorax
JT  - Thorax
JID - 0417353
RN  - 0 (Biomarkers)
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
SB  - IM
MH  - Adult
MH  - Biomarkers
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cell Count
MH  - Chemokine CCL2/*metabolism
MH  - Chemokine CXCL1
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/*metabolism
MH  - Female
MH  - Forced Expiratory Volume/physiology
MH  - Growth Substances/*metabolism
MH  - Humans
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Macrophages/chemistry
MH  - Male
MH  - Middle Aged
MH  - Neutrophils/chemistry
MH  - Pulmonary Disease, Chronic Obstructive/*etiology/metabolism
MH  - Smoking/metabolism
MH  - Sputum/cytology/*metabolism
PMC - PMC1746378
EDAT- 2002/07/04 10:00
MHDA- 2002/08/02 10:01
PMCR- 2005/07/01
CRDT- 2002/07/04 10:00
PHST- 2002/07/04 10:00 [pubmed]
PHST- 2002/08/02 10:01 [medline]
PHST- 2002/07/04 10:00 [entrez]
PHST- 2005/07/01 00:00 [pmc-release]
AID - 10.1136/thorax.57.7.590 [doi]
PST - ppublish
SO  - Thorax. 2002 Jul;57(7):590-5. doi: 10.1136/thorax.57.7.590.