PMID- 12098787
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 0582-9879 (Print)
IS  - 0582-9879 (Linking)
VI  - 32
IP  - 2
DP  - 2000
TI  - Construction and Characterization of Recombinant Adenoviruses Expressing 
      Biologically Active Human Brain-derived Neurotrophic Factor and Neurotrophin-3.
PG  - 121-125
AB  - In order to deliver brain-derived neurotrophic factor(BDNF) and 
      neurotrophin-3(NT3) into CNS to prevent or reduce degeneration of CNS neurons in 
      human neurodegenerative diseases and neuron injuries, recombinant adenoviruses 
      Ad-BDNF and Ad-NT3 were constructed. BDNF and NT3 genes were inserted into an 
      E1-substituted adenovirus shuttle plasmid, respectively, and were driven by the 
      human cytomegalovirus immediate-early gene promoter/enhancer. After 
      cotransfection into 293 cells with the adenovirus plasmid pJM17, the recombinant 
      adenovirus Ad-BDNF and Ad-NT3 were propagated in 293 cells via homologous 
      recombination. After two rounds of CsCl centrifugation, the human recombinant 
      adenovirus Ad-BDNF and Ad-NT3 were obtained with titer of 1x10(12) and 8x10(11) 
      pfu/ml, respectively. To examine the expression of BDNF and NT3, HeLa cells were 
      infected with Ad-BDNF and Ad-NT3, respectively. RT-PCR, Western blot and ELISA 
      analysis results showed that BDNF and NT3 genes could be transcribed and 
      translated in Ad-BDNF and Ad-NT3 infected HeLa cells. And the culture media 
      containing 10% conditioned medium of Ad-BDNF and Ad-NT3 infected Hela cells could 
      induce the neurite outgrowth from E8 dorsal root ganglion neurons.
FAU - Chen, Qian
AU  - Chen Q
AD  - Institute of Basic Medical Sciences, Academy of Military Medical Sciences, 
      Beijing 100850, China. fanm@nic.bmi.ac.cn
FAU - Wang, Jia-Zheng
AU  - Wang JZ
FAU - Liu, Hong
AU  - Liu H
FAU - Wu, Yan
AU  - Wu Y
FAU - Fan, Ming
AU  - Fan M
LA  - eng
PT  - Journal Article
PL  - China
TA  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
JT  - Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
JID - 20730160R
EDAT- 2002/07/06 10:00
MHDA- 2002/07/06 10:00
CRDT- 2002/07/06 10:00
PHST- 2002/07/06 10:00 [pubmed]
PHST- 2002/07/06 10:00 [medline]
PHST- 2002/07/06 10:00 [entrez]
PST - ppublish
SO  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000;32(2):121-125.