PMID- 12098800
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 0582-9879 (Print)
IS  - 0582-9879 (Linking)
VI  - 32
IP  - 2
DP  - 2000
TI  - Inactivation Analysis of HcNPV Cysteine Protease Gene and Chitinase Gene.
PG  - 187-191
AB  - The fragment of Hyphantria cunea nuclear polyhedrosis virus (HcNPV), which 
      contains cysteine protease gene(CP) and chitinase gene (ChiA),was inserted into 
      plasmid PCRII to construct transfer vector pHcCVdel. Recombinant transfer vector 
      pHcCvpolh was constructed by inserting the fragment of HcNPV polyhedrin gene 
      (polh) into the EcoRI site of the pHcCVdel. By contransfection of the pHcCvpolh 
      and HcNPV-PTTH(+) DNA, which contained Bombyx mori prothoracicotropic hormone 
      gene (PTTH) into SPIM cells, the region from +76 downstream of ChiA gene 
      initiation codon to +20 downstream of CP gene initiation codon was substituted by 
      the polh gene, and the recombinant virus HcNPVpolh(+)CP(-)ChiA(-)PTTH(+) was 
      produced. The recombinant virus, in which CP gene and ChiA gene were inactive, 
      could express PTTH gene and form polyhedra. SPIM cells infected with the 
      recombinant virus revealed that CP and ChiA gene were not essential for viral 
      replication and had no significant effect on polyhedron formation, but the 
      infected cells survived 2 days more than those infected with HcNPV and 
      HcNPVPTTH(+). Therefore, the expression time of foreign gene may be prolonged 
      when CP gene and ChiA gene were inactivated.
FAU - Gong, Cheng-Liang
AU  - Gong CL
AD  - Shanghai Institute of Biochemistry, the Chinese Academy of Science, Shanghai 
      200031, China. xfwu@sunm.shcnc.ac.cn
FAU - Kobayashi, Jun
AU  - Kobayashi J
FAU - Jin, Wei
AU  - Jin W
FAU - Wu, Xiang-Fu
AU  - Wu XF
LA  - eng
PT  - Journal Article
PL  - China
TA  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
JT  - Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
JID - 20730160R
EDAT- 2002/07/06 10:00
MHDA- 2002/07/06 10:00
CRDT- 2002/07/06 10:00
PHST- 2002/07/06 10:00 [pubmed]
PHST- 2002/07/06 10:00 [medline]
PHST- 2002/07/06 10:00 [entrez]
PST - ppublish
SO  - Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000;32(2):187-191.