PMID- 12099975
OWN - NLM
STAT- MEDLINE
DCOM- 20030121
LR  - 20191106
IS  - 1462-8902 (Print)
IS  - 1462-8902 (Linking)
VI  - 4
IP  - 4
DP  - 2002 Jul
TI  - Brain-derived neurotrophic factor ameliorates lipid metabolism in diabetic mice.
PG  - 262-9
AB  - AIM: It has been reported previously that brain-derived neurotrophic factor 
      (BDNF) regulates blood glucose metabolism in rodent obese diabetic models such as 
      C57BL/KsJ-leprdb/leprdb (db/db) mice. BDNF further regulates energy expenditure, 
      possibly through the central and autonomous nervous systems. In this study, we 
      evaluated the effect of BDNF on both lipid and glucose metabolisms to clarify its 
      action mechanism. METHODS: To control the energy intake, we used a pellet 
      pair-feeding apparatus to synchronize food intake precisely between BDNF-treated 
      and vehicle-treated db/db mice. BDNF (50 mg/kg/week) was subcutaneously injected 
      to male db/db mice twice weekly for 3 weeks, and blood glucose, serum biochemical 
      lipid parameters and tissue weights were measured. Liver triglyceride contents 
      were measured and liver sections were histologically analysed. RESULTS: Twice 
      weekly BDNF treatment for 3 weeks significantly lowered blood glucose compared 
      with pellet pair-fed, vehicle-treated db/db mice (294 +/- 109 vs. 529 +/- 91 
      mg/dL). Serum non-esterified free fatty acid (726 +/- 72 vs. 999 +/- 220 
      microEq/l), total cholesterol (125 +/- 8 vs. 151 +/- 23 mg/dL) and phospholipid 
      levels (215 +/- 13 vs. 257 +/- 36 mg/dL) of the BDNF-treated db/db mice decreased 
      significantly. Liver weights (1.51 +/- 0.11 vs. 2.05 +/- 0.11 g), liver 
      triglyceride contents (17.5 +/- 1.4 vs. 26.1 +/- 2.1 mg/g) and fatty liver in 
      histological appearance were reduced with BDNF treatment. There were no 
      significant differences in body weights and white adipose tissue weights between 
      the two groups. CONCLUSIONS: Taken together with the accelerating effect of BDNF 
      on energy metabolism, these findings indicate that BDNF improves glucose and 
      lipid metabolism in obese diabetic animals without enlarging liver or adipose 
      tissues.
FAU - Tsuchida, A
AU  - Tsuchida A
AD  - Sumitomo Pharmaceuticals Co. Ltd, Discovery Research Laboratories II, 
      Konohana-ku, Osaka 554-0022, Japan.
FAU - Nonomura, T
AU  - Nonomura T
FAU - Nakagawa, T
AU  - Nakagawa T
FAU - Itakura, Y
AU  - Itakura Y
FAU - Ono-Kishino, M
AU  - Ono-Kishino M
FAU - Yamanaka, M
AU  - Yamanaka M
FAU - Sugaru, E
AU  - Sugaru E
FAU - Taiji, M
AU  - Taiji M
FAU - Noguchi, H
AU  - Noguchi H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Diabetes Obes Metab
JT  - Diabetes, obesity & metabolism
JID - 100883645
RN  - 0 (Blood Glucose)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Adipose Tissue/drug effects/metabolism
MH  - Animals
MH  - Blood Glucose/drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cholesterol/blood
MH  - Crosses, Genetic
MH  - Diabetes Mellitus/*blood
MH  - *Lipid Metabolism
MH  - Liver/drug effects/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - *Obesity
MH  - Time Factors
EDAT- 2002/07/09 10:00
MHDA- 2003/01/22 04:00
CRDT- 2002/07/09 10:00
PHST- 2002/07/09 10:00 [pubmed]
PHST- 2003/01/22 04:00 [medline]
PHST- 2002/07/09 10:00 [entrez]
AID - 206 [pii]
AID - 10.1046/j.1463-1326.2002.00206.x [doi]
PST - ppublish
SO  - Diabetes Obes Metab. 2002 Jul;4(4):262-9. doi: 10.1046/j.1463-1326.2002.00206.x.