PMID- 12100164
OWN - NLM
STAT- MEDLINE
DCOM- 20020911
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 118
IP  - 1
DP  - 2002 Jul
TI  - All-trans retinoic acid prevents apoptosis of human marrow CD34+ cells deprived 
      of haematopoietic growth factors.
PG  - 289-95
AB  - The regulation of apoptosis plays a key role in haematopoiesis. It has been 
      demonstrated that haematopoietic progenitor cells progressively undergo apoptotic 
      cell death in the absence of appropriate growth factors. We studied the effects 
      of pharmacological doses of all-transretinoic acid (ATRA) on the apoptosis of 
      human adult marrow CD34+ progenitor cells cultured for 7 d in a serum-free 
      medium. We quantified CD34+ cells, clonogenic progenitors and 5 week 
      colony-forming cells (CFC) before and after ATRA exposure. Moreover, we defined 
      the apoptotic status of the CD34+ cell fraction by analysis of phosphatidylserine 
      externalization (using annexin V), the relative membrane permeability to 
      7-aminoactinomycin D (7AAD) and the mitochondrial membrane potential [using 
      3,3'-dihexyloxacarbocyanine iodide, DiOC6(3)]. In the drastic experimental 
      conditions used, a decrease in viable CD34+ cells, granulocyte-macrophage 
      colony-forming units (CFU-GM), erythroid burst-forming units (BFU-E) and 5 week 
      CFC were observed. Exposure to ATRA partially prevented the decrease in viable 
      CD34+, without a concomitant effect on the clonogenic and more immature 
      progenitors. ATRA-treated CD34+ cells displayed changes in apoptotic status 
      compared with control cultures, particularly in lower annexin V-binding. These 
      results were confirmed using 7AAD and DiOC6(3). Our results demonstrate that ATRA 
      exerts a protective effect on CD34+ cells exposed to such apoptotic stress.
FAU - Herault, Olivier
AU  - Herault O
AD  - EA-3249 'Cellules Hematopoietiques, Hemostase et Greffe' Faculty of Medicine, 
      University Hospital, Tours, France. olivier.herault@med.univ-tours.fr
FAU - Domenech, Jorge
AU  - Domenech J
FAU - Georget, Marie Therese
AU  - Georget MT
FAU - Clement, Nathalie
AU  - Clement N
FAU - Colombat, Philippe
AU  - Colombat P
FAU - Binet, Christian
AU  - Binet C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Annexins)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Culture Media, Serum-Free)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Annexins/metabolism
MH  - *Antigens, CD34
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Bone Marrow Cells/*drug effects/immunology/metabolism
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Clone Cells
MH  - Culture Media, Serum-Free
MH  - Humans
MH  - Protein Binding
MH  - Time Factors
MH  - Tretinoin/*pharmacology
EDAT- 2002/07/09 10:00
MHDA- 2002/09/12 10:01
CRDT- 2002/07/09 10:00
PHST- 2002/07/09 10:00 [pubmed]
PHST- 2002/09/12 10:01 [medline]
PHST- 2002/07/09 10:00 [entrez]
AID - 3573 [pii]
AID - 10.1046/j.1365-2141.2002.03573.x [doi]
PST - ppublish
SO  - Br J Haematol. 2002 Jul;118(1):289-95. doi: 10.1046/j.1365-2141.2002.03573.x.