PMID- 12100866
OWN - NLM
STAT- MEDLINE
DCOM- 20020924
LR  - 20190910
IS  - 0378-5173 (Print)
IS  - 0378-5173 (Linking)
VI  - 241
IP  - 2
DP  - 2002 Jul 25
TI  - A method for the incorporation of ovalbumin into immune stimulating complexes 
      prepared by the hydration method.
PG  - 385-9
AB  - This study describes the development of a method for the incorporation of 
      fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes 
      (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was 
      performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with 
      subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both 
      pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and 
      other non-liposomal colloidal structures. The degree of incorporation of 
      pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if 
      ISCOMs are the predominant colloids in the system.
FAU - Konnings, Stephanie
AU  - Konnings S
AD  - Colloidal Drug Delivery Group, School of Pharmacy, University of Otago, P.O. Box 
      913, Dunedin, New Zealand.
FAU - Copland, Melissa J
AU  - Copland MJ
FAU - Davies, Nigel M
AU  - Davies NM
FAU - Rades, Thomas
AU  - Rades T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Quillaja Saponins)
RN  - 0 (Saponins)
RN  - 66594-14-7 (Quil A)
RN  - 9006-59-1 (Ovalbumin)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adjuvants, Immunologic/administration & dosage/chemistry
MH  - Drug Delivery Systems/*methods
MH  - Fluorescein-5-isothiocyanate/*administration & dosage/chemistry
MH  - Fluorescent Dyes/administration & dosage/chemistry
MH  - Ovalbumin/*administration & dosage/chemistry
MH  - Quillaja Saponins
MH  - Saponins/administration & dosage/chemistry
EDAT- 2002/07/09 10:00
MHDA- 2002/09/25 06:00
CRDT- 2002/07/09 10:00
PHST- 2002/07/09 10:00 [pubmed]
PHST- 2002/09/25 06:00 [medline]
PHST- 2002/07/09 10:00 [entrez]
AID - S0378517302002703 [pii]
AID - 10.1016/s0378-5173(02)00270-3 [doi]
PST - ppublish
SO  - Int J Pharm. 2002 Jul 25;241(2):385-9. doi: 10.1016/s0378-5173(02)00270-3.