PMID- 12106332
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 4
IP  - 6
DP  - 1992
TI  - Expression of Ciliary Neurotrophic Factor and the Neurotrophins-Nerve Growth 
      Factor, Brain-Derived Neurotrophic Factor and Neurotrophin 3-in Cultured Rat 
      Hippocampal Astrocytes.
PG  - 459-471
AB  - Cultured astrocytes are known to possess a range of neurotrophic activities in 
      culture. In order to examine which factors may be responsible for these 
      activities, we have examined the expression of the genes for four known 
      neurotrophic factors-ciliary neurotrophic factor (CNTF), nerve growth factor 
      (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT3)-in 
      purified astrocyte cultures derived from neonatal rat hippocampus. Hippocampal 
      astrocytes were found to express mRNA for three neurotrophic factors-CNTF, NGF 
      and NT3-at significantly higher levels than other cultured cell types or cell 
      lines examined. BDNF messenger RNA (mRNA), however, was undetectable in these 
      astrocytes. The levels of CNTF, NGF and NT3 mRNA in astrocytes were largely 
      unaffected by their degree of confluency, while serum removal caused only a 
      transient decrease in mRNA levels, which returned to basal levels within 48 h. 
      Astrocyte-derived CNTF was found to comigrate with recombinant rat CNTF at 23 kD 
      on a Western blot. Immunocytochemical analysis revealed strong CNTF 
      immunoreactivity in the cytoplasm of astrocytes, weak staining in the nucleus, 
      but no CNTF at the cell surface. NGF and NT3 were undetectable 
      immunocytochemically. CNTF-like activity, as assessed by bioassay on ciliary 
      ganglion neurons, was found in the extract of cultured astrocytes but not in 
      conditioned medium, whereas astrocyte-conditioned medium supported survival of 
      dorsal root ganglion neurons but not ciliary or nodose ganglion neurons. This 
      conditioned medium activity was neutralized with antibodies to NGF. Astrocyte 
      extract also supported survival of dorsal root ganglion and nodose ganglion 
      neurons, but these activities were not blocked by anti-NGF. Part, but not all, of 
      the activity in astrocyte extracts which sustained nodose ganglion neurons could 
      be attributed to CNTF.
FAU - Rudge, John S
AU  - Rudge JS
AD  - Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, NY 10591, 
      USA.
FAU - Alderson, Ralph F
AU  - Alderson RF
FAU - Pasnikowski, Elizabeth
AU  - Pasnikowski E
FAU - McClain, Joyce
AU  - McClain J
FAU - Ip, Nancy Y
AU  - Ip NY
FAU - Lindsay, Ronald M
AU  - Lindsay RM
LA  - eng
PT  - Journal Article
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
EDAT- 1992/01/01 00:00
MHDA- 2002/07/11 10:00
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 2002/07/11 10:00 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - ejn_04060459 [pii]
AID - 10.1111/j.1460-9568.1992.tb00896.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 1992;4(6):459-471. doi: 10.1111/j.1460-9568.1992.tb00896.x.