PMID- 12106657
OWN - NLM
STAT- MEDLINE
DCOM- 20020911
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 504
IP  - 1-2
DP  - 2002 Jul 25
TI  - Induction of aneuploidy in male mouse germ cells detected by the sperm-FISH 
      assay: a review of the present data base.
PG  - 173-82
AB  - Multicolour fluorescence in situ hybridization (FISH) with chromosome-specific 
      DNA-probes can be used to assess aneuploidy (disomy) and diploidy in sperm of any 
      species provided the DNA-probes are available. In the present EU research 
      project, DNA-probes for mouse chromosomes 8, X and Y were employed each labelled 
      with different colours. Male mice were treated with the test chemicals and sperm 
      were sampled from the Caudae epididymes 22-24 days later to allow spermatocytes 
      exposed during meiosis to develop into mature sperm. At present, the data base 
      comprises 10 chemicals: acrylamide (AA), carbendazim (CB), colchicine (COL), 
      diazepam (DZ), griseofulvin (GF), omeprazole (OM), taxol (TX), thiobendazole 
      (TB), trichlorfon (TF) and vinblastine (VBL). Of these, COL and TF induced 
      disomic sperm only. DZ and GF induced disomic and diploid sperm, while CB and TB 
      induced diploid sperm only. VBL gave contradictory results in repeated 
      experiments in an inter-laboratory comparison. AA, OM and TX did not induce an 
      increase in disomic or diploid sperm at the doses used. The induction of 
      aneuploidy by DZ was also tested in humans. Sperm samples from patients after 
      attempted suicide and from patients with chronic Valium((R)) abuse were evaluated 
      using human DNA-probes specific for chromosomes 1,16, 21, X and Y. A quantitative 
      comparison between mouse and man indicates that male meiosis in humans is 10-100 
      times more sensitive than in mice to aneuploidy induction by DZ. The positive 
      response of mice to TF supports the hypothesis by Czeizel et al. [Lancet 341 
      (1993) 539] that TF may be causally related to the occurrence of congenital 
      abnormality clusters in a Hungarian village.
FAU - Adler, I-D
AU  - Adler ID
AD  - Institute of Experimental Genetics, GSF-National Research Center for Environment 
      and Health, Ingolstaedter Landstr. 1, Neuherberg, Germany. adler@gsf.de
FAU - Schmid, T E
AU  - Schmid TE
FAU - Baumgartner, A
AU  - Baumgartner A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Benzimidazoles)
RN  - 0 (Carbamates)
RN  - 20R035KLCI (Acrylamide)
RN  - 32HRV3E3D5 (Griseofulvin)
RN  - 5V9KLZ54CY (Vinblastine)
RN  - DBF2DG4G2K (Trichlorfon)
RN  - H75J14AA89 (carbendazim)
RN  - KG60484QX9 (Omeprazole)
RN  - N1Q45E87DT (Thiabendazole)
RN  - P88XT4IS4D (Paclitaxel)
RN  - Q3JTX2Q7TU (Diazepam)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Acrylamide/toxicity
MH  - *Aneuploidy
MH  - Animals
MH  - Benzimidazoles/toxicity
MH  - *Carbamates
MH  - Colchicine/toxicity
MH  - Diazepam/adverse effects/toxicity
MH  - Griseofulvin/toxicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Meiosis/drug effects/genetics
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Omeprazole/toxicity
MH  - Paclitaxel/toxicity
MH  - Spermatozoa/drug effects/*metabolism
MH  - Substance-Related Disorders/etiology/genetics
MH  - Thiabendazole/toxicity
MH  - Trichlorfon/toxicity
MH  - Vinblastine/toxicity
EDAT- 2002/07/11 10:00
MHDA- 2002/09/12 10:01
CRDT- 2002/07/11 10:00
PHST- 2002/07/11 10:00 [pubmed]
PHST- 2002/09/12 10:01 [medline]
PHST- 2002/07/11 10:00 [entrez]
AID - S0027510702000908 [pii]
AID - 10.1016/s0027-5107(02)00090-8 [doi]
PST - ppublish
SO  - Mutat Res. 2002 Jul 25;504(1-2):173-82. doi: 10.1016/s0027-5107(02)00090-8.