PMID- 12106696
OWN - NLM
STAT- MEDLINE
DCOM- 20021106
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 103
IP  - 1-2
DP  - 2002 Jun 30
TI  - Effect of ischemic preconditioning on the expression of putative neuroprotective 
      genes in the rat brain.
PG  - 106-15
AB  - Previous studies have demonstrated that sublethal ischemic insults protect from 
      subsequent ischemia in the intact brain. There are two windows for the induction 
      of tolerance by ischemic preconditioning (IPC). One occurs within 1 h following 
      IPC, and the other one develops from 1 to 3 days after IPC. The goal of this 
      study was to determine whether IPC neuroprotection may be mediated by expression 
      of known neuroprotective genes and to characterize the temporal and spatial 
      expression patterns of these genes. IPC was produced by bilateral carotid artery 
      occlusions and hypotension (50 mmHg) for 2 min. After various survival times, the 
      expression of MAP-2, brain-derived neurotrophic factor (BDNF), c-jun, c-fos, 
      nerve growth factor (NGF) and HSP70 was assessed by in situ hybridization of 
      coronal brain sections with 35S labeled probes. BDNF, NGF, and c-jun were 
      significantly upregulated in the hippocampus. c-fos was detected in the 
      hippocampus, cortex and striatum. HSP70 mRNA was induced in the cortex, 
      hippocampus, striatum, and thalamus. MAP-2 showed no change in expression, 
      confirming previous studies that no cell death occurs following IPC. The increase 
      in expression of these stress-related, neurotrophic and immediate early genes in 
      response to a mild preconditioning insult may help mediate the protection of 
      vulnerable neurons to subsequent lethal ischemic insults.
CI  - Copyright 2002 Elsevier Science B.V.
FAU - Truettner, Jessie
AU  - Truettner J
AD  - The Cerebral Vascular Disease Research Center, Department of Neurology (D4-5), 
      University of Miami School of Medicine, P.O. Box 16960, Miami, FL 33101, USA.
FAU - Busto, Raul
AU  - Busto R
FAU - Zhao, Weizhao
AU  - Zhao W
FAU - Ginsberg, Myron D
AU  - Ginsberg MD
FAU - Perez-Pinzon, Miguel A
AU  - Perez-Pinzon MA
LA  - eng
GR  - 35AIS30027/AI/NIAID NIH HHS/United States
GR  - 3P01NS05820/NS/NINDS NIH HHS/United States
GR  - 5R29NS34774-04/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain Chemistry/*genetics
MH  - Brain Ischemia/*physiopathology
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Gene Expression
MH  - Genes, Immediate-Early/physiology
MH  - HSP70 Heat-Shock Proteins/genetics
MH  - In Situ Hybridization
MH  - *Ischemic Preconditioning
MH  - Male
MH  - Microtubule-Associated Proteins/genetics
MH  - Nerve Growth Factor/genetics
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - Proto-Oncogene Proteins c-jun/genetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
EDAT- 2002/07/11 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/07/11 10:00
PHST- 2002/07/11 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/07/11 10:00 [entrez]
AID - S0169328X02001912 [pii]
AID - 10.1016/s0169-328x(02)00191-2 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2002 Jun 30;103(1-2):106-15. doi: 
      10.1016/s0169-328x(02)00191-2.