PMID- 12108790
OWN - NLM
STAT- MEDLINE
DCOM- 20021220
LR  - 20191025
IS  - 0743-5800 (Print)
IS  - 0743-5800 (Linking)
VI  - 28
IP  - 1-2
DP  - 2002 Feb-May
TI  - Investigation of red blood cell carbonic anhydrase, glucose 6-phosphate 
      dehydrogenase, hexokinase enzyme activities, and zinc concentration in patients 
      with hyperthyroid diseases.
PG  - 61-8
AB  - We have recently reported on the activities of glucose-6-phosphate dehydrogenase 
      (G6PD), hexokinase (HK) and carbonic anhydrase I (CA-I) and II (CA-II) isoenzymes 
      obtained from erythrocytes of healthy subjects and untreated patients with 
      hyperthyroid diseases. Also, erythrocyte zinc concentrations were measured. Red 
      blood cell (RBC) zinc (Zn) concentration was measured by atomic absorption 
      spectrophotometry. Activities of carbonic anhydrase II and I isoenzymes were 
      determined with CO2-hydratase activity method by using selective inactivation 
      with bromopyruvate. G6PD and HK enzyme activities were measured 
      spectrophotometrically via absorbance change (at 340 nm) in NADPH formed as a 
      result of the reactions catalysed by these enzymes. In statistical analysis of 
      all these parameters, activity of CA-I was 4388 +/- 207 (EU/gHb) and 2881 +/- 869 
      (EU/gHb) in healthy and untreated hyperthyroid subjects, respectively. The 
      activity values for CA-II were 5391 +/- 257 (EU/gHb) and 4688 +/- 12.6 (EU/gHb) 
      in healthy and untreated hyperthyroid subjects. Glucose 6-phosphate dehydrogenase 
      (G6PD) activity was 10.19 +/- 1.87 (EU/gHb) in healthy group and 4.92 +/- 2.49 
      (EU/gHb) in patient group. While hexokinase enzyme activity was 1.575 +/- 0.898 
      in healthy subjects, it was 0.651 +/- 0.418 (EU/gHb) in the patient group. While 
      erythrocyte zinc concentration in the healthy subjects was 49.32 +/- 23.5 
      (mg/gHb), this concentration for patients with uncontrolled hyperthyroid diseases 
      was significantly decreased to 29.62 +/- 4.26 (mg/gHb). As a conclusion, CA-I 
      isoenzyme, G6PD, hexokinase activities and erythrocyte zinc concentration had 
      decreased in untreated patients carrying hyperthyroid diseases as compared to 
      those of the healthy subjects.
FAU - Odcikin, Ebru
AU  - Odcikin E
AD  - Department of Biochemistry Medical School, Ataturk University, Erzurum, Turkey.
FAU - Ozdemir, Hasan
AU  - Ozdemir H
FAU - Ciftci, Mehmet
AU  - Ciftci M
FAU - Capoglu, Ilyas
AU  - Capoglu I
LA  - eng
PT  - Journal Article
PL  - England
TA  - Endocr Res
JT  - Endocrine research
JID - 8408548
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 53-59-8 (NADP)
RN  - 9002-71-5 (Thyrotropin)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 4.2.1.- (Carbonic Anhydrase I)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - J41CSQ7QDS (Zinc)
RN  - Q51BO43MG4 (Thyroxine)
SB  - IM
EIN - Endocr Res 2002 Aug;28(3):271
MH  - Adult
MH  - Carbonic Anhydrase I/blood
MH  - Carbonic Anhydrase II/blood
MH  - Carbonic Anhydrases/*blood
MH  - Erythrocytes/chemistry/*enzymology
MH  - Glucosephosphate Dehydrogenase/*blood
MH  - Hexokinase/*blood
MH  - Humans
MH  - Hyperthyroidism/*enzymology
MH  - Middle Aged
MH  - NADP/metabolism
MH  - Reference Values
MH  - Thyrotropin/blood
MH  - Thyroxine/blood
MH  - Triiodothyronine/blood
MH  - Zinc/*blood
EDAT- 2002/07/11 10:00
MHDA- 2002/12/21 04:00
CRDT- 2002/07/11 10:00
PHST- 2002/07/11 10:00 [pubmed]
PHST- 2002/12/21 04:00 [medline]
PHST- 2002/07/11 10:00 [entrez]
AID - 10.1081/erc-120004538 [doi]
PST - ppublish
SO  - Endocr Res. 2002 Feb-May;28(1-2):61-8. doi: 10.1081/erc-120004538.