PMID- 12109048
OWN - NLM
STAT- MEDLINE
DCOM- 20020729
LR  - 20141120
IS  - 0004-4172 (Print)
IS  - 0004-4172 (Linking)
VI  - 52
IP  - 6
DP  - 2002
TI  - Effects of gold-chloroquine complexes on respiratory burst of polymorphonuclear 
      leukocytes.
PG  - 468-74
AB  - The effects of gold-chloroquine derivatives with the formula [Au(PR3)(CQ)]PF6 
      (where R = Ph (1), Et or Me) on the superoxide anion production by human 
      neutrophils (PMNs, polymorphonuclear cells) were investigated. When these 
      complexes (0.1-3 mumol/l) were added to PMNs prior to the activators 
      formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), 
      they inhibited isoluminol-horseradish peroxidase-dependent chemiluminescence 
      (CLisol). The inhibition was a direct result of effects on PMNs since 
      chemiluminescence in the cell-free system (horseradish peroxidase-hydrogen 
      peroxidase-isoluminol) was not affected. The above mentioned concentrations of 
      the complexes did not show in vitro toxicity on the cells. On the other hand, 
      when 1 mumol/l of complex 1 was added to cells after stimulation, the 
      chemiluminescence of PMNs stimulated by PMA was inhibited, but not the 
      chemiluminescence stimulated by fMLP. The gold-chloroquine binding was essential 
      for the referred activity as chemiluminescence was not influenced by the 
      precursors chloroquine (CAS 54-05-7) and AuCl(PPh3). Furthermore, the extent of 
      inhibition of chemiluminescence in PMNs activated by PMA did not increase with 
      the duration of preincubation in presence of 1 mumol/l of 1. Extensive washing of 
      cells after preincubation with 1 mumol/l of 1 reversed the aforementioned 
      inhibition. All these results show that the gold-chloroquine binding can lead to 
      compounds with specific properties that could make them useful in the treatment 
      of some inflammatory diseases.
FAU - Navarro, Maribel
AU  - Navarro M
AD  - Chemistry Center, Instituto Venezolano de Investigaciones Cientificas (IVIC), 
      Caracas, Venezuela. mnavarro@ivic.ve
FAU - Vasquez, Flor
AU  - Vasquez F
FAU - Velasquez, Mait
AU  - Velasquez M
FAU - Medina, Rafael
AU  - Medina R
FAU - Reggio, Ronald
AU  - Reggio R
FAU - Fraile, German
AU  - Fraile G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Arzneimittelforschung
JT  - Arzneimittel-Forschung
JID - 0372660
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 7440-57-5 (Gold)
RN  - 886U3H6UFF (Chloroquine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Cell Survival/drug effects
MH  - Cell-Free System
MH  - Chloroquine/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Gold/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Luminescent Measurements
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*metabolism
MH  - Respiratory Burst/*drug effects
MH  - Superoxides/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 2002/07/12 10:00
MHDA- 2002/07/30 10:01
CRDT- 2002/07/12 10:00
PHST- 2002/07/12 10:00 [pubmed]
PHST- 2002/07/30 10:01 [medline]
PHST- 2002/07/12 10:00 [entrez]
AID - 10.1055/s-0031-1299916 [doi]
PST - ppublish
SO  - Arzneimittelforschung. 2002;52(6):468-74. doi: 10.1055/s-0031-1299916.