PMID- 12110696
OWN - NLM
STAT- MEDLINE
DCOM- 20030108
LR  - 20220310
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 20
IP  - 4
DP  - 2002
TI  - Bone marrow-derived stem cells can differentiate into retinal cells in injured 
      rat retina.
PG  - 279-83
AB  - It has recently been shown that bone marrow cells can differentiate into various 
      lineage cells including neural cells in vitro and in vivo. We therefore examined 
      whether bone marrow stem cells can differentiate into retinal neural cells in 
      adult rats. PKH-67-labeled stem cell-enriched bone marrow cells (BMCs) were 
      injected into the vitreous space of eyes in which the retinas had been 
      mechanically injured using a hooked needle. Two weeks after the injection of 
      these cells, immunohistochemical examinations were carried out. The stem 
      cell-enriched BMCs had been incorporated and had differentiated into retinal 
      neural cells in the injured retina. The stem cell-enriched BMCs had accumulated 
      mainly in the outer nuclear layer around the injured sites. The incorporated 
      cells expressed glial fibrillary acidic protein, calbindin, rhodopsin, and 
      vimentin. These results raise the possibility that stem cell-enriched BMCs have 
      the ability to differentiate into retinal neural cells, and that the injection of 
      stem cell-enriched BMCs into the retina would help repair damaged retinal cells.
FAU - Tomita, Minoru
AU  - Tomita M
AD  - First Department of Pathology, Kansai Medical University, Osaka, Japan.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Yamada, Haruhiko
AU  - Yamada H
FAU - Takahashi, Kanji
AU  - Takahashi K
FAU - Kiuchi, Katsuji
AU  - Kiuchi K
FAU - Oyaizu, Haruki
AU  - Oyaizu H
FAU - Ikebukuro, Kazuya
AU  - Ikebukuro K
FAU - Kaneda, Hiroyuki
AU  - Kaneda H
FAU - Matsumura, Miyo
AU  - Matsumura M
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Calbindins)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 0 (Vimentin)
RN  - 9009-81-8 (Rhodopsin)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Amacrine Cells/cytology/metabolism
MH  - Animals
MH  - Astrocytes/cytology/*metabolism
MH  - Calbindins
MH  - Cell Differentiation/*physiology
MH  - Cell Movement/physiology
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Graft Survival/physiology
MH  - Hematopoietic Stem Cell Transplantation/*methods/trends
MH  - Hematopoietic Stem Cells/cytology/*metabolism
MH  - Immunohistochemistry
MH  - Leukocyte Common Antigens/metabolism
MH  - Male
MH  - Neurons/cytology/*metabolism
MH  - Rats
MH  - Retina/cytology/injuries/*surgery
MH  - Retinal Diseases/*therapy
MH  - Retinal Rod Photoreceptor Cells/cytology/metabolism
MH  - Rhodopsin/metabolism
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Vimentin/metabolism
EDAT- 2002/07/12 10:00
MHDA- 2003/01/09 04:00
CRDT- 2002/07/12 10:00
PHST- 2002/07/12 10:00 [pubmed]
PHST- 2003/01/09 04:00 [medline]
PHST- 2002/07/12 10:00 [entrez]
AID - 10.1634/stemcells.20-4-279 [doi]
PST - ppublish
SO  - Stem Cells. 2002;20(4):279-83. doi: 10.1634/stemcells.20-4-279.