PMID- 12110700
OWN - NLM
STAT- MEDLINE
DCOM- 20030108
LR  - 20131121
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 20
IP  - 4
DP  - 2002
TI  - Treatment of Shwachman syndrome by Japanese herbal medicine (Juzen-taiho-to): 
      stimulatory effects of its fatty acids on hemopoiesis in patients.
PG  - 311-9
AB  - Juzen-taiho-to (a Japanese herbal medicine) has been traditionally administered 
      to patients with anemia, neutropenia, or wasting syndrome. We previously 
      attempted to isolate and purify the hemopoiesis-stimulatory components in 
      Juzen-taiho-to extracts using an in vitro hemopoietic stem cell (HSC) assay 
      method in which mouse HSCs can proliferate on a stromal cell line (MS-5). We have 
      found that fatty acids (particularly oleic acid and linolenic acid) actively 
      promote the proliferation of HSCs, and that the effect is mediated by stromal 
      cells, rather than by any direct action on the HSCs. In the present study, we 
      show, using human normal bone marrow cells (BMCs) and umbilical cord blood cells, 
      that similar stimulatory effects are due to the presence of oleic acid and 
      linolenic acid, which stimulate the proliferation of HSCs in stroma-based culture 
      systems. Furthermore, a marked stimulatory effect was noted on BMCs from patients 
      with Shwachman syndrome, which shows pancreatic and bone marrow dysfunctions. We 
      also show the data on hemopoietic recovery after the administration of 
      Juzen-taiho-to to a patient with Shwachman syndrome. These findings suggest that 
      decreased fatty acid levels in the blood, caused by exocrine pancreatic 
      insufficiency, induce bone marrow dysfunction in Shwachman syndrome.
FAU - Hisha, Hiroko
AU  - Hisha H
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Kohdera, Urara
AU  - Kohdera U
FAU - Hirayama, Masahiro
AU  - Hirayama M
FAU - Yamada, Haruki
AU  - Yamada H
FAU - Iguchi-Uehira, Tomoko
AU  - Iguchi-Uehira T
FAU - Fan, Tian-Xue
AU  - Fan TX
FAU - Cui, Yun-Ze
AU  - Cui YZ
FAU - Yang, Guo-Xiang
AU  - Yang GX
FAU - Li, Yongan
AU  - Li Y
FAU - Sugiura, Kikuya
AU  - Sugiura K
FAU - Inaba, Muneo
AU  - Inaba M
FAU - Kobayashi, Yohnosuke
AU  - Kobayashi Y
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Fatty Acids)
RN  - 0 (Mitogens)
RN  - 0 (juzentaihoto)
RN  - 0RBV727H71 (alpha-Linolenic Acid)
RN  - 2UMI9U37CP (Oleic Acid)
SB  - IM
MH  - Bone Marrow Diseases/*drug therapy/metabolism/physiopathology
MH  - Cell Division/*drug effects/physiology
MH  - Cells, Cultured
MH  - Child
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Fatty Acids/blood/*therapeutic use
MH  - Female
MH  - Hematopoiesis/*drug effects/physiology
MH  - Hematopoietic Stem Cells/cytology/*drug effects/metabolism
MH  - Humans
MH  - Male
MH  - Mitogens/*therapeutic use
MH  - Oleic Acid/pharmacology
MH  - Pancreatic Diseases/drug therapy/etiology/physiopathology
MH  - Pancytopenia/drug therapy/etiology/physiopathology
MH  - Recovery of Function/drug effects/physiology
MH  - Treatment Outcome
MH  - alpha-Linolenic Acid/pharmacology
EDAT- 2002/07/12 10:00
MHDA- 2003/01/09 04:00
CRDT- 2002/07/12 10:00
PHST- 2002/07/12 10:00 [pubmed]
PHST- 2003/01/09 04:00 [medline]
PHST- 2002/07/12 10:00 [entrez]
AID - 10.1634/stemcells.20-4-311 [doi]
PST - ppublish
SO  - Stem Cells. 2002;20(4):311-9. doi: 10.1634/stemcells.20-4-311.