PMID- 12111403
OWN - NLM
STAT- MEDLINE
DCOM- 20021204
LR  - 20041117
IS  - 0918-4287 (Print)
IS  - 0918-4287 (Linking)
VI  - 35
IP  - 1
DP  - 2002 Mar
TI  - Expression of matrix metalloproteinases, tissue inhibitors of metalloproteinase, 
      collagens, and Ki67 antigen in pleural malignant mesothelioma: an 
      immunohistochemical and electron microscopic study.
PG  - 16-23
AB  - Because matrix metalloproteinases (MMPs) degrade extracellular matrix, including 
      basement membrane, and because tissue inhibitors of MMP (TIMPs) suppress MMP 
      activities, MMPs and TIMPs are considered to play important roles in invasion and 
      metastasis in many malignancies. We examined immunohistochemically the expression 
      of MMPs (MMP-1, -2, -3, -7, and -9), TIMPs (TIMP-1 and -2), and collagens (types 
      I, III, and IV) in 16 patients with pleural malignant mesothelioma (PMM; 8 with 
      the epithelial, 4 with the sarcomatous, and 4 with the biphasic type). Electron 
      microscopy revealed that the tumor cells in all types possessed the 
      characteristics of malignant mesotheliomas, including numerous microvilli and 
      moderate amounts of intermediate filaments. Basement lamina was present only 
      focally. The proliferative Ki67 index was at a high level, compared with values 
      reported in various other malignancies. Positive staining for MMP-1 was observed 
      in most tumor cells in all 16 patients (100%). MMP-2 was expressed in most tumor 
      cells in 2 patients (13%). In contrast, MMP-3, -7, and -9 were not detected in 
      any PMM. TIMP-1 and TIMP-2 were expressed in 3 patients (19%) and 2 patients 
      (13%), respectively. The stromal cells were simultaneously positive for MMPs or 
      TIMPs in the patients whose tumor parenchymal cells were positive for each 
      enzyme. These results indicate that the expression of MMP-1 and MMP-2 may be 
      related to PMM invasion and spread. In particular, as MMP-1 was overexpressed in 
      contrast to the lower expression of TIMP-1, MMP-1 is strongly suggested to play 
      an important role in PMM invasion by degrading the tumor stroma. In spite of 
      general agreement that epithelial-type PMM has a better prognosis than other 
      types, there was no significant difference in the Ki67 index among the 
      histological types of PMM.
FAU - Hirano, Hiroshi
AU  - Hirano H
AD  - Department of Pathology, Osaka Medical College, Osaka, Japan. 
      patho@toneyama.hosp.go.jp
FAU - Tsuji, Motomu
AU  - Tsuji M
FAU - Kizaki, Tomohiko
AU  - Kizaki T
FAU - Sashikata, Terumasa
AU  - Sashikata T
FAU - Yoshi, Yasuyoshi
AU  - Yoshi Y
FAU - Okada, Yoshikatsu
AU  - Okada Y
FAU - Mori, Hiroshi
AU  - Mori H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Med Electron Microsc
JT  - Medical electron microscopy : official journal of the Clinical Electron 
      Microscopy Society of Japan
JID - 9430789
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Aged
MH  - Collagen/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Ki-67 Antigen/*metabolism
MH  - Male
MH  - Matrix Metalloproteinases/*metabolism
MH  - Mesothelioma/*metabolism/ultrastructure
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Mitotic Index
MH  - Pleural Neoplasms/*metabolism/ultrastructure
MH  - Tissue Inhibitor of Metalloproteinases/*metabolism
EDAT- 2002/07/12 10:00
MHDA- 2002/12/05 04:00
CRDT- 2002/07/12 10:00
PHST- 2002/07/12 10:00 [pubmed]
PHST- 2002/12/05 04:00 [medline]
PHST- 2002/07/12 10:00 [entrez]
AID - 10.1007/s007950200002 [doi]
PST - ppublish
SO  - Med Electron Microsc. 2002 Mar;35(1):16-23. doi: 10.1007/s007950200002.