PMID- 12111797
OWN - NLM
STAT- MEDLINE
DCOM- 20020814
LR  - 20061115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 69
IP  - 2
DP  - 2002 Jul 15
TI  - Brain-derived neurotrophic factor applied to the motor cortex promotes sprouting 
      of corticospinal fibers but not regeneration into a peripheral nerve transplant.
PG  - 160-8
AB  - Previous experiments from our laboratory have shown that application of 
      brain-derived neurotrophic factor (BDNF) to the red nucleus or the motor cortex 
      stimulates an increase in the expression of regeneration-associated genes in 
      rubrospinal and corticospinal neurons. Furthermore, we have previously shown that 
      BDNF application stimulates regeneration of rubrospinal axons into a peripheral 
      graft after a thoracic injury. The current study investigates whether application 
      of BDNF to the motor cortex will facilitate regeneration of corticospinal neurons 
      into a peripheral nerve graft placed into the thoracic spinal cord. In adult 
      Sprague Dawley rats, the dorsal columns and the corticospinal tract between T9 
      and T10 were ablated by suction, and a 5-mm-long segment of predegenerated tibial 
      nerve was autograft implanted into the lesion. With an osmotic pump, BDNF was 
      infused directly into the parenchyma of the motor cortex for 14 days. Growth of 
      the corticospinal tract into the nerve graft was then evaluated by transport of 
      an anterograde tracer. Anterogradely labeled corticospinal fibers were not 
      observed in the peripheral nerve graft in animals treated with saline or BDNF. 
      Serotinergic and noradrenergic fibers, as well as peripheral sensory afferents, 
      were observed to penetrate the graft, indicating the viability of the peripheral 
      nerve graft as a permissive growth substrate for these specific fiber types. 
      Although treatment of the corticospinal fibers with BDNF failed to produce 
      regeneration into the graft, there was a distinct increase in the number of 
      axonal sprouts rostral to the injury site. This indicates that treatment of 
      corticospinal neurons with neurotrophins, e.g., BDNF, can be used to enhance 
      sprouting of corticospinal axons within the spinal cord. Whether such sprouting 
      leads to functional recovery after spinal cord injury is currently under 
      investigation.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Hiebert, G W
AU  - Hiebert GW
AD  - CORD (Collaboration On Repair Discoveries), University of British Columbia, 
      Vancouver, British Columbia, Canada. hiebert@cord.ubc.ca
FAU - Khodarahmi, K
AU  - Khodarahmi K
FAU - McGraw, J
AU  - McGraw J
FAU - Steeves, J D
AU  - Steeves JD
FAU - Tetzlaff, W
AU  - Tetzlaff W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology/*physiology
MH  - Immunohistochemistry
MH  - Male
MH  - Motor Cortex/*drug effects
MH  - Nerve Regeneration/*drug effects
MH  - Peripheral Nerves/*drug effects
MH  - Pyramidal Tracts/drug effects/*growth & development
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transplants
EDAT- 2002/07/12 10:00
MHDA- 2002/08/15 10:01
CRDT- 2002/07/12 10:00
PHST- 2002/07/12 10:00 [pubmed]
PHST- 2002/08/15 10:01 [medline]
PHST- 2002/07/12 10:00 [entrez]
AID - 10.1002/jnr.10275 [doi]
PST - ppublish
SO  - J Neurosci Res. 2002 Jul 15;69(2):160-8. doi: 10.1002/jnr.10275.