PMID- 12112583
OWN - NLM
STAT- MEDLINE
DCOM- 20030312
LR  - 20161124
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 62
IP  - 4
DP  - 2002 Aug
TI  - Two kinase activities are sufficient for sea urchin sperm chromatin 
      decondensation in vitro.
PG  - 496-503
AB  - Decondensation of compact and inactive sperm chromatin by egg cytoplasm at 
      fertilization is necessary to convert the male germ cell chromatin to an active 
      somatic form. We studied decondensation of sea urchin sperm nuclei in a cell-free 
      extract of sea urchin eggs to define conditions promoting decondensation. We find 
      that egg cytosol specifically phosphorylates two sperm-specific (Sp) histones in 
      vitro in the same regions as in vivo. This activity is blocked by olomoucine, an 
      inhibitor of cdc2-like kinases, but not by chelerythrine, an inhibitor of protein 
      kinase C (PKC). PKC phosphorylates and solubilizes the sperm nuclear lamina, one 
      requirement for decondensation. Olomoucine, which does not inhibit lamina 
      removal, blocks sperm nuclear decondensation in the same concentration range over 
      which it is effective in blocking Sp histone phosphorylation. In a system free of 
      other soluble proteins, neither PKC nor cdc2 alone elicit sperm chromatin 
      decondensation, but the two act synergistically to decondense sperm nuclei. We 
      conclude that two kinases activities are sufficient for sea urchin male 
      pronuclear decondensation in vitro, a lamin kinase (PKC) and a cdc2-like Sp 
      histone kinase.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Stephens, S
AU  - Stephens S
AD  - Department of Biology, Amherst College, Amherst, Massachusetts 01002, USA.
FAU - Beyer, B
AU  - Beyer B
FAU - Balthazar-Stablein, U
AU  - Balthazar-Stablein U
FAU - Duncan, R
AU  - Duncan R
FAU - Kostacos, M
AU  - Kostacos M
FAU - Lukoma, M
AU  - Lukoma M
FAU - Green, G R
AU  - Green GR
FAU - Poccia, D
AU  - Poccia D
LA  - eng
GR  - GM-55970/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Alkaloids)
RN  - 0 (Benzophenanthridines)
RN  - 0 (Chromatin)
RN  - 0 (Histones)
RN  - 0 (Phenanthridines)
RN  - 0 (Purines)
RN  - 6A839B2HYS (olomoucine)
RN  - E3B045W6X0 (chelerythrine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - P39Y9652YJ (Kinetin)
SB  - IM
MH  - Alkaloids
MH  - Animals
MH  - Benzophenanthridines
MH  - CDC2 Protein Kinase/chemistry/*metabolism
MH  - Chromatin/*metabolism
MH  - Enzyme Stability
MH  - Histones/metabolism
MH  - Kinetin
MH  - Male
MH  - Ovum/metabolism
MH  - Phenanthridines/metabolism
MH  - Phosphorylation
MH  - Protein Kinase C/chemistry/*metabolism
MH  - Purines/metabolism
MH  - Sea Urchins/*enzymology/genetics
MH  - Spermatozoa/cytology/*enzymology
EDAT- 2002/07/12 10:00
MHDA- 2003/03/13 04:00
CRDT- 2002/07/12 10:00
PHST- 2002/07/12 10:00 [pubmed]
PHST- 2003/03/13 04:00 [medline]
PHST- 2002/07/12 10:00 [entrez]
AID - 10.1002/mrd.90005 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2002 Aug;62(4):496-503. doi: 10.1002/mrd.90005.