PMID- 12114732
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1559-0097 (Electronic)
IS  - 1046-3976 (Linking)
VI  - 7
IP  - 3
DP  - 1996 Autumn
TI  - Interphase Analysis of Chromosome 11 in Human Pituitary Somatotroph Adenomas by 
      Direct Fluorescence In Situ Hybridization.
PG  - 203-206
AB  - Chromosome 11 numerical abnormalities were detected by fluorescence in situ 
      hybridization (FISH) technique in four surgically removed pituitary somatotroph 
      adenomas from patients clinically associated with acromegaly. The tumors were 
      diagnosed by histology. immunocytochemistry, and electron microscopy, and 
      included two densely granulated somatotroph (DG-SM) and two sparsely granulated 
      somatotroph (SG-SM) adenomas. For demonstration of chromosome 11, the direct FISH 
      technique was applied on imprints from fresh adenoma tissue fixed in acetone 
      using an a-satellite specific centromeric probe. The slides were studied with a 
      fluorescence microscope and the percentages of positive nuclei with aberrant 
      fluorescent signals were counted. All tumors exhibited numerical chromosomal 
      abnormalities in 8-23% of their cell population and included nuclei containing 
      1-3 extra chromosome 11 copies. The SC-SM adenomas exhibited more prominent 
      abnormalities compared with the DG-SM adenomas. We conclude that numerical 
      chromosome 11 abnormalities represent a frequent event among somatotroph adenomas 
      with a tendency for higher frequency in SC-SM adenomas.
FAU - Kontogeorgos, George
AU  - Kontogeorgos G
FAU - Kapranos, Nikiforos
AU  - Kapranos N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocr Pathol
JT  - Endocrine pathology
JID - 9009288
EDAT- 1996/10/01 00:00
MHDA- 2002/07/13 10:00
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 2002/07/13 10:00 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - EP0703203 [pii]
AID - 10.1007/BF02739922 [doi]
PST - ppublish
SO  - Endocr Pathol. 1996 Autumn;7(3):203-206. doi: 10.1007/BF02739922.