PMID- 12115452
OWN - NLM
STAT- MEDLINE
DCOM- 20030304
LR  - 20061115
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 61
IP  - 4
DP  - 2002 Sep 15
TI  - The effect of polyethylene particle phagocytosis on the viability of mature human 
      macrophages.
PG  - 619-27
AB  - Macrophages are the major cell type observed in the inflammatory membrane 
      retrieved at implant revision surgery. In this study, mature human 
      monocyte-derived macrophages (MDM) were adapted to a previously established in 
      vitro model to examine the influence of high-density polyethylene (HDPE) 
      particulate (4-10 microm) on MDM viability. HDPE particles were suspended in 
      soluble type I collagen, which subsequently was solidified on glass coverslips. 
      Mature human macrophages, derived from differentiating peripheral blood monocytes 
      on polystyrene for 10 days, were incubated in culture media on collagen controls 
      and collagen-particle substrata for 31 days. Histologic analysis demonstrated 
      that MDMs were in contact with the particles at 2 h. The majority of the 
      particles were associated with the cells within 24 h. Based on electron 
      microscopy, those cells associated with the particles appeared to be 
      morphologically activated rather than necrotic or apoptotic. Assessment of cell 
      viability revealed no differences among the groups at 24 h, but at 31 days 
      significantly more viable cells and higher DNA values were found associated with 
      the particle groups versus the collagen controls. The histologic results validate 
      human mature MDMs as a clinically relevant cell type for study of the role of 
      polyethylene particulate in aseptic loosening. The cell viability results 
      indicate that phagocytosis of HDPE is not toxic to MDMs but in fact prolongs MDM 
      survival. The long-lived MDMs may play a role in perpetuating chronic 
      inflammation surrounding implants.
CI  - Copyright 2002 Wiley Periodicals, Inc.
FAU - Xing, S
AU  - Xing S
AD  - Institute of Biomaterials and Biomedical Engineering, University of Toronto, 170 
      College Street, Toronto, Ontario, Canada M5S 3E3.
FAU - Santerre, J P
AU  - Santerre JP
FAU - Labow, R S
AU  - Labow RS
FAU - Boynton, E L
AU  - Boynton EL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Collagen Type I)
RN  - 9002-88-4 (Polyethylene)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Separation
MH  - *Cell Survival
MH  - Cells, Cultured
MH  - Collagen Type I/metabolism
MH  - DNA/analysis
MH  - Flow Cytometry
MH  - Humans
MH  - Macrophages/*physiology/ultrastructure
MH  - Particle Size
MH  - *Phagocytosis
MH  - Polyethylene/*metabolism
MH  - Prostheses and Implants
EDAT- 2002/07/13 10:00
MHDA- 2003/03/05 04:00
CRDT- 2002/07/13 10:00
PHST- 2002/07/13 10:00 [pubmed]
PHST- 2003/03/05 04:00 [medline]
PHST- 2002/07/13 10:00 [entrez]
AID - 10.1002/jbm.10078 [doi]
PST - ppublish
SO  - J Biomed Mater Res. 2002 Sep 15;61(4):619-27. doi: 10.1002/jbm.10078.