PMID- 12115949
OWN - NLM
STAT- MEDLINE
DCOM- 20020805
LR  - 20091119
IS  - 0148-639X (Print)
IS  - 0148-639X (Linking)
VI  - 26
IP  - 1
DP  - 2002 Jul
TI  - IGF-I, IGF-II, and IGF-receptor-1 transcript and IGF-II protein expression in 
      myostatin knockout mice tissues.
PG  - 55-63
AB  - Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and 
      immunohistochemistry were performed to demonstrate whether a correlation exists 
      between insulin-like growth factors (IGFs)-positive regulators of growth-and 
      myostatin, a negative regulator of muscle growth. IGF-I, -II, and IGF-receptor-1 
      (IGF-R1) mRNA and IGF-II protein expressions were determined in control and 
      myostatin knockout mice tissues. IGF-I gene expressions were similar between 
      control and knockout mice tissues, whereas IGF-II mRNA levels were significantly 
      higher in myostatin knockout mice kidney and soleus muscles than those of control 
      mice (P <.01). IGF-R1 mRNA levels from control mice heart (P <.05) and kidney (P 
      <.01) were significantly higher than in myostatin knockout mice, whereas levels 
      were lower in pectoralis muscle of control mice than knockout mice (P <.01). The 
      strongly IGF-II-positive cells in soleus muscle were more common in myostatin 
      knockout mice and were seen in a few foci in control mice. IGF-II 
      immunoreactivity in both control and myostatin knockout mice kidneys was 
      localized to the epithelium of renal tubules and collecting ducts. Reciprocal 
      changes in the expression of myostatin and IGF-II and IGF-R1 may underlie normal 
      growth of skeletal muscle and other organs in mammals, and the changes in these 
      tissues associated with disease.
CI  - Copyright 2002 Wiley Periodicals, Inc.
FAU - Kocamis, Hakan
AU  - Kocamis H
AD  - Division of Histology and Embryology, Kafkas University, Kars, Turkey.
FAU - Gahr, Scott A
AU  - Gahr SA
FAU - Batelli, Lori
AU  - Batelli L
FAU - Hubbs, Ann F
AU  - Hubbs AF
FAU - Killefer, John
AU  - Killefer J
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Muscle Nerve
JT  - Muscle & nerve
JID - 7803146
RN  - 0 (Actins)
RN  - 0 (Mstn protein, mouse)
RN  - 0 (Myostatin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - Actins/genetics/metabolism
MH  - Animals
MH  - Brain/metabolism
MH  - Immunohistochemistry
MH  - Insulin-Like Growth Factor I/*genetics/metabolism
MH  - Insulin-Like Growth Factor II/genetics/*metabolism
MH  - Kidney/cytology/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Muscle, Skeletal/cytology/metabolism
MH  - Myocardium/metabolism
MH  - Myostatin
MH  - Organ Specificity
MH  - RNA, Messenger/*metabolism
MH  - Receptor, IGF Type 1/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transforming Growth Factor beta/*deficiency/genetics
EDAT- 2002/07/13 10:00
MHDA- 2002/08/06 10:01
CRDT- 2002/07/13 10:00
PHST- 2002/07/13 10:00 [pubmed]
PHST- 2002/08/06 10:01 [medline]
PHST- 2002/07/13 10:00 [entrez]
AID - 10.1002/mus.10160 [doi]
PST - ppublish
SO  - Muscle Nerve. 2002 Jul;26(1):55-63. doi: 10.1002/mus.10160.