PMID- 12115952
OWN - NLM
STAT- MEDLINE
DCOM- 20020805
LR  - 20131121
IS  - 0148-639X (Print)
IS  - 0148-639X (Linking)
VI  - 26
IP  - 1
DP  - 2002 Jul
TI  - Macrophage apoptosis in rat skeletal muscle treated with bupivacaine 
      hydrochloride: possible role of MCP-1.
PG  - 79-86
AB  - The fate of macrophages infiltrating damaged rat skeletal muscle fibers after 
      intramuscular injection of the anesthetic bupivacaine hydrochloride (BPVC) and 
      the possible roles of monocyte chemoattractant protein-1 (MCP-1) were 
      investigated. The number of macrophages reached a maximum level at 2 days after 
      the injection and then gradually decreased. The number of apoptotic cells 
      detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling 
      (TUNEL) assay was elevated at 2-4 days and decreased thereafter. In serial 
      sections, TUNEL-positive cells were also immunopositive for RM-4, an antibody 
      specific for identification of macrophages. Electron microscopy further confirmed 
      that it was the macrophages themselves that were undergoing apoptosis. As 
      assessed by reverse transcriptase-polymerase chain reaction (RT-PCR), high levels 
      of MCP-1 mRNA in BPVC-treated muscles were observed and positively correlated 
      with maximum macrophage infiltration. However, the levels of MCP-1 mRNA returned 
      to normal low values coincident with decrease of inflammation and healing of 
      damaged muscle fiber. Local apoptosis of macrophages, under the control of MCP-1, 
      may be involved in healing of BPVC-treated muscles.
CI  - Copyright 2002 Wiley Periodicals, Inc.
FAU - Horiguchi, Taisuke
AU  - Horiguchi T
AD  - Department of Orthopedic Surgery, Osaka Medical College, Takatsuki, Osaka, Japan.
FAU - Shibata, Masa-Aki
AU  - Shibata MA
FAU - Ito, Yuko
AU  - Ito Y
FAU - Eid, Nabil A S
AU  - Eid NA
FAU - Abe, Muneaki
AU  - Abe M
FAU - Otsuki, Yoshinori
AU  - Otsuki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Muscle Nerve
JT  - Muscle & nerve
JID - 7803146
RN  - 0 (Anesthetics, Local)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - Y8335394RO (Bupivacaine)
SB  - IM
MH  - Anesthetics, Local/administration & dosage/pharmacology
MH  - Animals
MH  - *Apoptosis
MH  - Bupivacaine/administration & dosage/*pharmacology
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Inflammation/chemically induced/pathology
MH  - Injections, Intramuscular
MH  - Lipopolysaccharides/pharmacology
MH  - Lung/drug effects/metabolism/pathology
MH  - Macrophages/*metabolism/pathology/ultrastructure
MH  - Male
MH  - Microscopy, Electron
MH  - Muscle Fibers, Skeletal/drug effects/pathology/ultrastructure
MH  - Muscle, Skeletal/*drug effects/pathology/ultrastructure
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/drug effects/metabolism/pathology
EDAT- 2002/07/13 10:00
MHDA- 2002/08/06 10:01
CRDT- 2002/07/13 10:00
PHST- 2002/07/13 10:00 [pubmed]
PHST- 2002/08/06 10:01 [medline]
PHST- 2002/07/13 10:00 [entrez]
AID - 10.1002/mus.10162 [doi]
PST - ppublish
SO  - Muscle Nerve. 2002 Jul;26(1):79-86. doi: 10.1002/mus.10162.