PMID- 12115999
OWN - NLM
STAT- MEDLINE
DCOM- 20020828
LR  - 20181130
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 67
IP  - 4
DP  - 2002 Aug
TI  - HHV-6 infects human aortic and heart microvascular endothelial cells, increasing 
      their ability to secrete proinflammatory chemokines.
PG  - 528-33
AB  - Endothelial cells are important targets for herpesvirus infection. To evaluate 
      the biological effects of human herpesvirus-6 (HHV-6) infection, adult heart 
      microvascular and aortic endothelial cells were examined for in vitro 
      susceptibility to HHV-6 and for the alterations induced by viral infection on the 
      production of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 
      (IL-8). Analysis by reverse transcription-polymerase chain reaction and by in 
      situ polymerase chain reaction showed that HHV-6 replicates in endothelium in the 
      absence of cytopathic effects, and that viral sequences were present in 20% 
      umbilical vein and in 10% aortic and 1% microvascular endothelium. HHV-6 
      infection upregulated the production of MCP-1 and IL-8, with differences observed 
      between aortic and microvascular endothelium. These findings demonstrate that 
      endothelial cells represent a potential reservoir for HHV-6 infection, and the 
      altered pattern of chemokine production can lead to attraction of immunocompetent 
      cells and to the development of inflammatory processes.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Caruso, Arnaldo
AU  - Caruso A
AD  - Institute of Microbiology, University of Brescia Medical School, Italy.
FAU - Rotola, Antonella
AU  - Rotola A
FAU - Comar, Manola
AU  - Comar M
FAU - Favilli, Flavia
AU  - Favilli F
FAU - Galvan, Monica
AU  - Galvan M
FAU - Tosetti, Maria
AU  - Tosetti M
FAU - Campello, Cesare
AU  - Campello C
FAU - Caselli, Elisabetta
AU  - Caselli E
FAU - Alessandri, Giulio
AU  - Alessandri G
FAU - Grassi, Manuela
AU  - Grassi M
FAU - Garrafa, Emirena
AU  - Garrafa E
FAU - Cassai, Enzo
AU  - Cassai E
FAU - Di Luca, Dario
AU  - Di Luca D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Aorta/*virology
MH  - Cell Line
MH  - Chemokine CCL2/biosynthesis/metabolism
MH  - Chemokines/immunology/*metabolism
MH  - Endothelium, Vascular/immunology/*metabolism/*virology
MH  - Heart/*virology
MH  - Herpesviridae Infections/*immunology/*virology
MH  - Herpesvirus 6, Human/genetics/*physiology
MH  - Humans
MH  - Interleukin-8/biosynthesis/metabolism
MH  - Leukocytes/immunology
MH  - RNA, Viral/analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2002/07/13 10:00
MHDA- 2002/08/29 10:01
CRDT- 2002/07/13 10:00
PHST- 2002/07/13 10:00 [pubmed]
PHST- 2002/08/29 10:01 [medline]
PHST- 2002/07/13 10:00 [entrez]
AID - 10.1002/jmv.10133 [doi]
PST - ppublish
SO  - J Med Virol. 2002 Aug;67(4):528-33. doi: 10.1002/jmv.10133.