PMID- 12116338
OWN - NLM
STAT- MEDLINE
DCOM- 20021212
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 50
IP  - 3
DP  - 2002 Jun 15
TI  - Incidence of numerical chromosome aberrations in meningioma tumors as revealed by 
      fluorescence in situ hybridization using 10 chromosome-specific probes.
PG  - 153-9
AB  - OBJECTIVE: Although information on the cytogenetic characteristics of meningioma 
      tumors has accumulated progressively over the past few decades, information on 
      the genetic heterogeneity of meningiomas is still scanty. The aim of the present 
      study was to analyze by interphase fluorescence in situ hybridization (FISH) the 
      incidence of numerical abnormalities for chromosomes 1, 9, 10, 11, 14, 15, 17, 
      22, X, and Y in a group of 70 consecutive meningioma tumors. Another goal was to 
      establish the potential associations among the altered chromosomes, as a way to 
      assess both intertumoral and intratumoral heterogeneity. METHODS: For the purpose 
      of the study, 70 patients diagnosed with meningioma were analyzed. Interphase 
      FISH for the detection of numerical abnormalities for chromosomes 1, 9, 10, 11, 
      14, 15, 17, 22, X, and Y was applied to fresh tumor samples from each of the 
      patients studied. RESULTS: The overall incidence of numerical abnormalities was 
      76%. Chromosome Y in males and chromosome 22 in the whole series were the most 
      common abnormalities (46% and 61%, respectively). Despite the finding that 
      monosomy of chromosome 22/22q(-) deletions are the most frequent individual 
      abnormality (53%), we have observed that chromosome gains are significantly more 
      common than chromosome losses (60% versus 40%). Chromosome gains corresponded to 
      abnormalities of chromosomes 1 (27%), 9 (25%), 10 (23%), 11 (22%), 14 (33%), 15 
      (22%), 17 (23%), and X in females (35%) and males (23%) whereas chromosome losses 
      apart from chromosome 22 frequently involved chromosomes 14 (19%), X in males 
      (23%), and Y in males (32%). Although an association was found among most gained 
      chromosomes on one side and chromosome losses on the other side, different 
      association patterns were observed. Furthermore, in the latter group, monosomy 
      22/22q(-) was associated with monosomy X in females and monosomy 14/14q(-) was 
      associated with nulisomy Y in males. In addition, chromosome losses usually 
      involved a large proportion of the tumor cells whereas chromosome gains were 
      restricted to small tumor cell clones, including tetraploid cells. CONCLUSIONS: 
      Our results show that meningiomas are genetically heterogeneous tumors that 
      display different patterns of numerical chromosome changes, as assessed by 
      interphase FISH.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Sayagues, Jose Maria
AU  - Sayagues JM
AD  - Servicio General de Citometria, Departmento de Medicina y Centro de 
      Investigaciones del Cancer, Universidad de Salamanca, Paseo de San Vicente, 
      58-182 37007 Salamanca, Spain.
FAU - Tabernero, Maria Dolores
AU  - Tabernero MD
FAU - Maillo, Angel
AU  - Maillo A
FAU - Diaz, Pedro
AU  - Diaz P
FAU - Rasillo, Ana
AU  - Rasillo A
FAU - Bortoluci, Aglae
AU  - Bortoluci A
FAU - Gomez-Moreta, Juan
AU  - Gomez-Moreta J
FAU - Santos-Briz, Angel
AU  - Santos-Briz A
FAU - Morales, Francisco
AU  - Morales F
FAU - Orfao, Alberto
AU  - Orfao A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adolescent
MH  - Aged
MH  - *Chromosome Aberrations/classification/statistics & numerical data
MH  - Chromosomes, Human
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Incidence
MH  - Male
MH  - Meningeal Neoplasms/diagnosis/*genetics/pathology
MH  - Meningioma/diagnosis/*genetics/pathology
EDAT- 2002/07/13 10:00
MHDA- 2002/12/13 04:00
CRDT- 2002/07/13 10:00
PHST- 2002/07/13 10:00 [pubmed]
PHST- 2002/12/13 04:00 [medline]
PHST- 2002/07/13 10:00 [entrez]
AID - 10.1002/cyto.10075 [doi]
PST - ppublish
SO  - Cytometry. 2002 Jun 15;50(3):153-9. doi: 10.1002/cyto.10075.