PMID- 12117258
OWN - NLM
STAT- MEDLINE
DCOM- 20021018
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 65
IP  - 7
DP  - 2002 Jul
TI  - Changes in the antigenic and immunoglobulin E-binding properties of hen's egg 
      albumin with the combination of heat and gamma irradiation treatment.
PG  - 1192-5
AB  - This study was carried out to evaluate the changes in the allergenic and 
      antigenic properties of hen's egg albumin (ovalbumin [OVA]) with the combination 
      of heat and gamma irradiation treatment. OVA solution samples were treated by (i) 
      heating (sample 1), (ii) irradiation after heating (sample 2), and (iii) heating 
      after irradiation (sample 3). Samples were isothermally heated and irradiated at 
      the absorption dose of 10 kGy. Competitive indirect enzyme-linked immunosorbent 
      assays (ELISAs) were performed with blood serum to test the ability of treated 
      OVA to bind to immunoglobulin E (IgE) and mouse murine monoclonal antibody (IgG). 
      OVA's ability to bind to mouse IgG changed upon heating at 75 degrees C, and its 
      ability to bind to egg-allergic IgE changed upon heating at 80 degrees C. The 
      ELISAs showed that egg-allergic IgE did not recognize OVA very well when heated 
      at > or = 80 degrees C, while mouse IgG retained better activity under these 
      conditions. Egg-allergic IgE binding was low both for OVA samples treated by 
      heating and for samples treated by irradiation followed by heating. These results 
      show that allergies induced by OVA could be effectively reduced by the 
      combination of heat and gamma irradiation treatment.
FAU - Kim, Mi-Jung
AU  - Kim MJ
AD  - Department of Food Science and Nutrition, Anyang University, Korea.
FAU - Lee, Ju-Woon
AU  - Lee JW
FAU - Yook, Hong-Sun
AU  - Yook HS
FAU - Lee, Soo-Young
AU  - Lee SY
FAU - Kim, Myung-Chul
AU  - Kim MC
FAU - Byun, Myung-Woo
AU  - Byun MW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (Egg Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Binding, Competitive
MH  - Egg Proteins/*immunology/radiation effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Food Hypersensitivity/*prevention & control
MH  - Gamma Rays
MH  - Hot Temperature
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Immunoglobulin G/*immunology
MH  - Mice
MH  - Ovalbumin/*immunology/radiation effects
MH  - Protein Denaturation
EDAT- 2002/07/16 10:00
MHDA- 2002/10/19 04:00
CRDT- 2002/07/16 10:00
PHST- 2002/07/16 10:00 [pubmed]
PHST- 2002/10/19 04:00 [medline]
PHST- 2002/07/16 10:00 [entrez]
AID - S0362-028X(22)04971-7 [pii]
AID - 10.4315/0362-028x-65.7.1192 [doi]
PST - ppublish
SO  - J Food Prot. 2002 Jul;65(7):1192-5. doi: 10.4315/0362-028x-65.7.1192.