PMID- 12118155
OWN - NLM
STAT- MEDLINE
DCOM- 20021106
LR  - 20190910
IS  - 0300-4864 (Print)
IS  - 0300-4864 (Linking)
VI  - 30
IP  - 8
DP  - 2001 Aug
TI  - Peroxisome proliferator-activated receptors (PPARs) and peroxisomes in rat 
      cortical and cerebellar astrocytes.
PG  - 671-83
AB  - Astrocytes are the most versatile cells of the neural tissue. Numerous astrocytic 
      functions--such as protection from oxidative damage, catabolism of neuroactive 
      D-amino acids acting as neuromodulators, synthesis and catabolism of some lipid 
      molecules, and, possibly, gluconeogenesis--reside in peroxisomes. The expression 
      of several peroxisomal enzymes, particularly those of the acyl-CoA beta-oxidation 
      pathway, is regulated by a class of ligand-activated transcription factors, known 
      as peroxisome proliferator-activated receptors (PPARs), acting on their target 
      genes as heterodimers with the retinoid X receptors (RXRs). In this work, primary 
      and secondary cultures of astrocytes from the cerebral cortices and cerebella of 
      neonatal rats (2 and 7 days of postnatal age) were utilized to investigate the 
      expression of peroxisomal enzymes, PPAR and RXR isotypes (alpha, beta and gamma), 
      by both biochemical and immunological methods. The results obtained demonstrate 
      that astrocytes in vitro express peroxisomal enzymes, PPARs, and RXRs and that 
      differences dependent on brain area, animal age, and culture time are reminiscent 
      of the in vivo situation. Therefore, primary cultures of astrocytes and, 
      particularly, high purified subcultures may constitute a useful model for further 
      studies aimed to gain further insights into the roles of peroxisomes and PPARs 
      related to lipid and glucose metabolism in these cells.
FAU - Cristiano, L
AU  - Cristiano L
AD  - Department of Basic and Applied Biology, University of L'Aquila, via Vetoio, 
      67010 Coppito (AQ), Italy.
FAU - Bernardo, A
AU  - Bernardo A
FAU - Ceru, M P
AU  - Ceru MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurocytol
JT  - Journal of neurocytology
JID - 0364620
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.3.3.6 (Acyl-CoA Oxidase)
RN  - EC 1.4.3.3 (D-Amino-Acid Oxidase)
SB  - IM
MH  - Acyl-CoA Oxidase
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/cytology/*enzymology
MH  - Catalase/metabolism
MH  - Cell Compartmentation/physiology
MH  - Cells, Cultured
MH  - Cerebellar Cortex/cytology/*enzymology
MH  - Cerebral Cortex/cytology/*enzymology
MH  - D-Amino-Acid Oxidase/metabolism
MH  - Fluorescent Antibody Technique
MH  - Oxidoreductases/metabolism
MH  - Peroxisomes/*enzymology
MH  - Protein Isoforms/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Receptors, Retinoic Acid/metabolism
MH  - Retinoid X Receptors
MH  - Transcription Factors/*metabolism
EDAT- 2002/07/16 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/07/16 10:00
PHST- 2002/07/16 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/07/16 10:00 [entrez]
AID - 10.1023/a:1016525716209 [doi]
PST - ppublish
SO  - J Neurocytol. 2001 Aug;30(8):671-83. doi: 10.1023/a:1016525716209.