PMID- 12119132
OWN - NLM
STAT- MEDLINE
DCOM- 20020911
LR  - 20190819
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 133
IP  - 2-3
DP  - 2002 Jul 21
TI  - Expression of hsp 90 in the human kidney and in proximal tubule cells exposed to 
      heat, sodium arsenite and cadmium chloride.
PG  - 241-54
AB  - The expression of heat shock protein (hsp) 90alpha and beta mRNA and protein were 
      determined in the human kidney and in human proximal tubule (HPT) cells exposed 
      to lethal and sub-lethal concentrations of Cd(+2) under both acute and extended 
      conditions of exposure. Using immunohistochemical analysis, it was demonstrated 
      that hsp 90 was widely distributed in the human adult and fetal kidney. Moderate 
      to strong staining was observed in the straight portions of the distal and 
      proximal tubules, the distal convoluted tubule, the collecting ducts and the 
      parietal epithelium of Bowmans capsule in the glomerulus. Moderate staining was 
      observed in the proximal convoluted tubule of the cortex and the thick loops of 
      Henle within the medulla. In addition, the fetal kidney demonstrated strong 
      staining of the blastema, the 'S-shaped' bodies, and the developing glomeruli. 
      Analysis of hsp 90alpha and beta mRNA expression in total RNA isolated from in 
      situ microdissected proximal tubules or HPT cells demonstrated similar expression 
      levels of both the alpha and beta isoforms in this tubule segment. It was 
      demonstrated that HPT cells exhibited the classic heat shock response when 
      subjected to a physical (heat) or chemical stress (NaAsO(2)). Heat stress, 
      elevated temperature at 42.5 degrees C for 1 h, caused a modest increase in both 
      hsp 90alpha and beta mRNA and protein. Similar results were obtained when the 
      cells were subjected to a classic chemical stress of exposure to 100 microM 
      NaAsO(2) for 4 h. In contrast, acute exposure of HPT cells to 53.4 microM CdCl(2) 
      for 4 h resulted in no consistent increase in hsp 90alpha and beta mRNA or 
      protein. Chronic exposure to Cd(+2) likewise failed to increase either hsp 90 
      mRNA or protein expression, even at concentrations of Cd(+2) that were lethal to 
      the cells during the time course. This study shows that the HPT has a high basal 
      expression of hsp 90, which is not induced by Cd(+2) exposure.
FAU - Somji, Seema
AU  - Somji S
AD  - Robert C. Byrd Health Sciences Center, Department of Surgery, Program in Genetics 
      and Developmental Biology, West Virginia University, Morgantown, USA. 
      ssomji@hsc.wvu.edu
FAU - Ann Sens, Mary
AU  - Ann Sens M
FAU - Garrett, Scott H
AU  - Garrett SH
FAU - Gurel, Volkan
AU  - Gurel V
FAU - Todd, John H
AU  - Todd JH
FAU - Sens, Donald A
AU  - Sens DA
LA  - eng
GR  - ES10039/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Arsenites)
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (RNA, Messenger)
RN  - 00BH33GNGH (Cadmium)
RN  - N5509X556J (arsenite)
SB  - IM
MH  - Arsenites/*toxicity
MH  - Blotting, Western
MH  - Cadmium/*toxicity
MH  - Cell Survival
MH  - Cells, Cultured
MH  - HSP90 Heat-Shock Proteins/*biosynthesis
MH  - Hot Temperature/*adverse effects
MH  - Humans
MH  - Immunohistochemistry
MH  - Isomerism
MH  - Kidney/drug effects/*metabolism
MH  - Kidney Tubules, Proximal/drug effects/*metabolism
MH  - Paraffin Embedding
MH  - RNA, Messenger/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2002/07/18 10:00
MHDA- 2002/09/12 10:01
CRDT- 2002/07/18 10:00
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2002/09/12 10:01 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - S0378427402002059 [pii]
AID - 10.1016/s0378-4274(02)00205-9 [doi]
PST - ppublish
SO  - Toxicol Lett. 2002 Jul 21;133(2-3):241-54. doi: 10.1016/s0378-4274(02)00205-9.