PMID- 12119230
OWN - NLM
STAT- MEDLINE
DCOM- 20020812
LR  - 20191106
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 166
IP  - 2
DP  - 2002 Jul 15
TI  - Analysis of nitric oxide synthase and nitrotyrosine expression in human pulmonary 
      tuberculosis.
PG  - 178-86
AB  - The role of nitric oxide (NO) in the host-defense against human tuberculosis (TB) 
      is controversial. Although experimental evidence indicates that NO may play an 
      important role in controlling TB, its expression in human tuberculous lungs has 
      not been systematically characterized. We therefore investigated the expression 
      of NO synthases (NOS) and of nitrotyrosine, the latter a marker of NO expression, 
      in surgically resected lungs of eight patients with TB. Immunohistochemical and 
      morphometric analyses revealed that, compared with control subjects, inducible 
      NOS, endothelial NOS, and nitrotyrosine, but not neuronal NOS, were significantly 
      elevated in the inflammatory zone of the tuberculous granulomas, and in the 
      nongranulomatous pneumonitis zone. Tumor necrosis factor-alpha (TNF-alpha) was 
      also significantly increased in tuberculous lungs and was principally localized 
      to the necrotic, and to a lesser extent, the inflammatory and fibrotic areas of 
      the granulomas. The NOS isoforms, nitrotyrosine, and TNF-alpha were expressed by 
      the epithelioid macrophages and giant cells within the granulomas and in alveolar 
      macrophages and epithelial cells in pneumonitis areas. This descriptive study 
      provides evidence that in human TB, NOS isoenzymes and NO are present in 
      specialized areas of the tuberculous granulomas; their precise role in human TB 
      remains to be determined.
FAU - Choi, Hyung-Seok
AU  - Choi HS
AD  - Department of Medicine, Program in Cell Biology, National Jewish Medical and 
      Research Center, Denver, Colorado 80206, USA.
FAU - Rai, Pradeep R
AU  - Rai PR
FAU - Chu, Hong Wei
AU  - Chu HW
FAU - Cool, Carlyne
AU  - Cool C
FAU - Chan, Edward D
AU  - Chan ED
LA  - eng
GR  - HL-66-112/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3604-79-3 (3-nitrotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - EC 1.14.13.39 (NOS1 protein, human)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
CIN - Am J Respir Crit Care Med. 2002 Jul 15;166(2):130-1. PMID: 12119220
CIN - Am J Respir Crit Care Med. 2003 Jun 15;167(12):1718; author reply 1718. PMID: 
      12796058
MH  - Epithelium/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Lung/*metabolism/pathology
MH  - Lymphocytes/pathology
MH  - Macrophages/metabolism/pathology
MH  - Macrophages, Alveolar/metabolism/pathology
MH  - Nitric Oxide Synthase/*metabolism
MH  - Nitric Oxide Synthase Type I
MH  - Nitric Oxide Synthase Type II
MH  - Nitric Oxide Synthase Type III
MH  - Tuberculosis, Pulmonary/*metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Tyrosine/*analogs & derivatives/*metabolism
EDAT- 2002/07/18 10:00
MHDA- 2002/08/13 10:01
CRDT- 2002/07/18 10:00
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2002/08/13 10:01 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - 10.1164/rccm.2201023 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2002 Jul 15;166(2):178-86. doi: 10.1164/rccm.2201023.