PMID- 12120710
OWN - NLM
STAT- MEDLINE
DCOM- 20020906
LR  - 20191106
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 110
IP  - 3
DP  - 2002 Jun
TI  - Immunohistochemical study on pulpal response in rat molars after cavity 
      preparation by Er:YAG laser.
PG  - 237-45
AB  - While Er:YAG laser systems are in extensive use for caries removal and cavity 
      preparation, the effects of such treatment on pulp tissue remain unclear. This 
      study evaluates these systems using immunohistochemical methods and compares the 
      results with information gained from treatment using conventional burs. Cervical 
      cavities were prepared in the upper first molars of rats, using either an Er:YAG 
      laser or a conventional tungsten-carbide bur. At intervals of 5 min, 6 h, 12 h, 1 
      d, 3 d and 7 d after cavity preparation, the teeth were processed for 
      immunohistochemical analyses of tissue non-specific alkaline phosphatase, 
      OX6-positive major histocompatibility complex class II antigen-expressing cells 
      and PGP 9.5-immunoreactive nerve fibers. DNA fragmentation was detected by the 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      method. Tissue non-specific alkaline phosphatase was observed mainly in the 
      subodontoblastic layer under the cavity lesion, from 5 min, in both groups. The 
      immunoreactivity was more pronounced in the laser group, but by 7 d no 
      significant differences were recognizable. At 12 h, TUNEL-positive cells were 
      detected around the odontoblastic layer in both groups. From 3 d to 7 d, a 
      limited number of positive cells were still visible in the group that underwent 
      standard treatment. Clear similarities in the distribution patterns of 
      OX6-immunopositive cells and PGP 9.5-immunoreactive nerve fibers were also noted. 
      From 12 h to 1 d, OX6-positive cells accumulated along the pulp-dentin border, 
      extending their processes into the dentinal tubules. Numerous bead-like PGP 
      9.5-immunoreactive nerve fibers were observed under the odontoblastic layer at 7 
      d. These results demonstrated that there was no appreciable difference in the 
      manner in which pulp tissue responded to treatment with either Er:YAG laser or a 
      conventional drill. This would seem to indicate the usefulness of the Er:YAG 
      laser system in the removal of caries and cavity preparation.
FAU - Tanabe, Keita
AU  - Tanabe K
AD  - Department of Oral Health Science, Niigata University Graduate School of Medical 
      and Dental Sciences, Japan.
FAU - Yoshiba, Kunihiko
AU  - Yoshiba K
FAU - Yoshiba, Nagako
AU  - Yoshiba N
FAU - Iwaku, Masaaki
AU  - Iwaku M
FAU - Ozawa, Hidehiro
AU  - Ozawa H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Aluminum Silicates)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Tungsten Compounds)
RN  - 11130-73-7 (tungsten carbide)
RN  - 58784XQC3Y (Yttrium)
RN  - 77B218D3YE (Erbium)
RN  - EC 3.1.2.- (Thiolester Hydrolases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Alkaline Phosphatase/analysis
MH  - Aluminum Silicates
MH  - Animals
MH  - Antigen-Presenting Cells/pathology
MH  - DNA Fragmentation
MH  - Dental Cavity Preparation/instrumentation/*methods
MH  - Dental Pulp/*pathology
MH  - Dentin/pathology
MH  - Erbium
MH  - Fluorescent Antibody Technique, Direct
MH  - Histocompatibility Antigens Class II/analysis
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - *Laser Therapy
MH  - Male
MH  - Molar
MH  - Nerve Fibers/ultrastructure
MH  - Nerve Tissue Proteins/analysis
MH  - Odontoblasts/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Statistics as Topic
MH  - Thiolester Hydrolases/analysis
MH  - Time Factors
MH  - Tooth Cervix/surgery
MH  - Tungsten Compounds
MH  - Ubiquitin Thiolesterase
MH  - Yttrium
EDAT- 2002/07/18 10:00
MHDA- 2002/09/07 10:01
CRDT- 2002/07/18 10:00
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2002/09/07 10:01 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - 10.1034/j.1600-0722.2002.21282.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2002 Jun;110(3):237-45. doi: 10.1034/j.1600-0722.2002.21282.x.