PMID- 12121676
OWN - NLM
STAT- MEDLINE
DCOM- 20030130
LR  - 20220311
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 63
IP  - 8
DP  - 2002 Aug
TI  - Pediatric celiac disease in India is associated with multiple DR3-DQ2 haplotypes.
PG  - 677-82
AB  - The role of human leukocyte antigen (HLA) DQ2 heterodimer (DQA1*0501-DQB1*0201) 
      in presenting gluten peptides to effector T cells in celiac disease (CD) has been 
      well documented. Because HLA-DQ2 is carried on DR3 haplotypes due to linkage 
      disequilibrium, such haplotypes are encountered more frequently in patients with 
      autoimmune disease. This study analyzed 35 North Indian children below 15 years 
      of age and diagnosed to have CD as per the ESPGAN criteria, which included 
      histopathologic alterations in duodenal biopsies, clinical response to gluten 
      withdrawal, and presence of antiendomysial antibodies. The HLA class I and class 
      II alleles were determined by polymerase chain reaction-sequence-specific 
      primers, sequence-specific oligonucleotide probe, and reverse line strip 
      molecular techniques. A statistically significant positive association of the 
      disease with HLA-DRB1*03 (94.2% versus 22.1% in controls, chi(2) = 73.4, p = 
      7.54E-11), and a negative association with DRB1*15 (chi(2) = 7.4, p = 6.5E-03) 
      and DRB1*13 alleles was observed. The HLA-DQB1*0201 was observed in all the 35 
      patients (100%), whereas the DQ2 heterodimer alpha(0)beta(0) occurred in 97.1% of 
      CD patients (31.4% in double dose, 65.7% in single dose) and revealed significant 
      deviation from healthy controls (chi(2) = 102.08, p = 7.56E-11). Further analysis 
      revealed involvement of multiple DR3+ve haplotypes with CD in Indians, of which 
      A26-B8-DR3 was the most common DR3 haplotype among patients (34.28%, chi(2) = 
      40.57, p = 2.65E-10) followed by Ax-B21-DR3 (11.4%) (chi(2) = 13.8, p = 2E-04) 
      and the classical Caucasian haplotype A1-B8-DR3 (5.7%). The former two haplotypes 
      are characteristic of Asian Indians and are involved in the development of CD. We 
      conclude that the high risk DR3 haplotypes that play a crucial role in the 
      development of CD are unique in Asian Indians. Detailed analysis of these 
      haplotypes in Indian patients with autoimmune diseases may help understand the 
      influence of other intervening genes within the major histocompatibility complex.
FAU - Kaur, Gurvinder
AU  - Kaur G
AD  - Department of Transplant Immunology and Immunogenetics, All India Institute of 
      Medical Sciences, New Delhi, India.
FAU - Sarkar, N
AU  - Sarkar N
FAU - Bhatnagar, S
AU  - Bhatnagar S
FAU - Kumar, S
AU  - Kumar S
FAU - Rapthap, C C
AU  - Rapthap CC
FAU - Bhan, M K
AU  - Bhan MK
FAU - Mehra, N K
AU  - Mehra NK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ2 antigen)
RN  - 0 (HLA-DR3 Antigen)
SB  - IM
MH  - Adolescent
MH  - Alleles
MH  - Celiac Disease/*genetics/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Gene Frequency
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DR3 Antigen/*genetics
MH  - Haplotypes
MH  - Humans
MH  - India
MH  - Infant
MH  - Male
EDAT- 2002/07/18 10:00
MHDA- 2003/01/31 04:00
CRDT- 2002/07/18 10:00
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2003/01/31 04:00 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - S0198885902004135 [pii]
AID - 10.1016/s0198-8859(02)00413-5 [doi]
PST - ppublish
SO  - Hum Immunol. 2002 Aug;63(8):677-82. doi: 10.1016/s0198-8859(02)00413-5.