PMID- 12121768
OWN - NLM
STAT- MEDLINE
DCOM- 20021022
LR  - 20190910
IS  - 0162-0134 (Print)
IS  - 0162-0134 (Linking)
VI  - 91
IP  - 1
DP  - 2002 Jul 25
TI  - NMR studies on interaction of lauryl maltoside with cytochrome c oxidase: a model 
      for surfactant interaction with the membrane protein.
PG  - 116-24
AB  - Interaction of lauryl maltoside (LM) surfactant with bovine heart cytochrome c 
      oxidase (CcO) has been studied by NMR techniques. Detailed 2-D (1)H and (13)C NMR 
      techniques were used to assign the NMR signals of the surfactant nuclei. 
      Paramagnetic dipolar shift of the surfactant (13)C NMR signals were used to 
      identify the atoms close to the enzyme. The diamagnetic carbon monoxide complex 
      of CcO did not cause any shift in the surfactant NMR spectra suggesting that the 
      paramagnetic centres of the native CcO cause the shifts by dipolar interactions. 
      The results showed that the polar head groups of the surfactant comprised of two 
      maltoside rings are more affected, while the hydrophobic tail groups did not show 
      any significant change on binding of the surfactant to the enzyme. This indicated 
      that surfactant head groups possibly bind to the enzyme surface and the 
      hydrophobic tail of the surfactant forms micelles and remains away from the 
      enzyme. Based on the results, we propose that the membrane bound enzyme is 
      possibly stabilised in aqueous solution by association with the micelles of the 
      neutral surfactant so that the polar heads of the micelles bind to the polar 
      surface of the enzyme. These micelles might form a 'belt like' structure around 
      the enzyme helping it to remain monodispersed in the active form.
FAU - Chattopadhyay, Krishnananda
AU  - Chattopadhyay K
AD  - Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi 
      Bhabha Road, Colaba, Mumnai 400 005, India. krish@biochem.wustl.edu
FAU - Das, Tapan Kanti
AU  - Das TK
FAU - Majumdar, Ananya
AU  - Majumdar A
FAU - Mazumdar, Shyamalava
AU  - Mazumdar S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 0 (Detergents)
RN  - 0 (Glucosides)
RN  - 69227-93-6 (dodecyl maltoside)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Detergents/*chemistry
MH  - Electron Transport Complex IV/*chemistry/metabolism
MH  - Glucosides/*chemistry
MH  - Models, Chemical
MH  - Molecular Structure
MH  - Myocardium/*enzymology
MH  - Nuclear Magnetic Resonance, Biomolecular
EDAT- 2002/07/18 10:00
MHDA- 2002/10/31 04:00
CRDT- 2002/07/18 10:00
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - S0162013402004270 [pii]
AID - 10.1016/s0162-0134(02)00427-0 [doi]
PST - ppublish
SO  - J Inorg Biochem. 2002 Jul 25;91(1):116-24. doi: 10.1016/s0162-0134(02)00427-0.