PMID- 12121897
OWN - NLM
STAT- MEDLINE
DCOM- 20020815
LR  - 20181130
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 283
IP  - 2
DP  - 2002 Aug
TI  - In situ correlation of cytokine secretion and apoptosis in Helicobacter 
      pylori-associated gastritis.
PG  - G481-8
AB  - Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) are 
      important for the pathogenesis of Helicobacter pylori-associated gastritis and 
      peptic ulcer disease. Gastric biopsies from H. pylori-positive and -negative 
      patients were used to examine the in situ correlation of TNF-alpha and IFN-gamma 
      with epithelial cell apoptosis, bacterial load, and histological parameters of 
      gastritis. From the same patients, we isolated H. pylori-specific T cell lines 
      and clones and examined their ex vivo release of proinflammatory cytokines. We 
      found a highly significant correlation of TNF-alpha and IFN-gamma production with 
      activity and grade of gastritis (P < 0.01), H. pylori density (P = 0.01), 
      epithelial cell apoptosis (P < 0.001), and Fas/Fas-ligand expression (P < 0.001). 
      T cell lines and clones were all TCR-alphabeta(+) and showed T helper 1 
      functional phenotype. With the use of serial histological sections, this study 
      showed for the first time the in situ correlation of TNF-alpha and IFN-gamma with 
      epithelial cell apoptosis, bacterial load, and histological severity of disease 
      and emphasizes the role of these cytokines in the pathophysiology of H. 
      pylori-associated disease.
FAU - Lehmann, Frank Serge
AU  - Lehmann FS
AD  - Department of Gastroenterology, University Hospital of Basel, Switzerland.
FAU - Terracciano, Luigi
AU  - Terracciano L
FAU - Carena, Ilaria
AU  - Carena I
FAU - Baeriswyl, Christian
AU  - Baeriswyl C
FAU - Drewe, Jurgen
AU  - Drewe J
FAU - Tornillo, Luigi
AU  - Tornillo L
FAU - De Libero, Gennaro
AU  - De Libero G
FAU - Beglinger, Christoph
AU  - Beglinger C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Cytokines)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (fas Receptor)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Apoptosis
MH  - Cell Line
MH  - Clone Cells
MH  - Cytokines/*metabolism
MH  - Fas Ligand Protein
MH  - Gastric Mucosa/metabolism
MH  - Gastritis/*microbiology/pathology/*physiopathology
MH  - HLA-DR Antigens/metabolism
MH  - *Helicobacter Infections
MH  - *Helicobacter pylori
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Middle Aged
MH  - Stomach/pathology
MH  - T-Lymphocytes/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - fas Receptor/metabolism
EDAT- 2002/07/18 10:00
MHDA- 2002/08/16 10:01
CRDT- 2002/07/18 10:00
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2002/08/16 10:01 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - 10.1152/ajpgi.00422.2001 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2002 Aug;283(2):G481-8. doi: 
      10.1152/ajpgi.00422.2001.