PMID- 12122001
OWN - NLM
STAT- MEDLINE
DCOM- 20021113
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 39
DP  - 2002 Sep 27
TI  - Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing 
      nonintegrin (DC-SIGN, CD209), a C-type surface lectin in human DCs, is a receptor 
      for Leishmania amastigotes.
PG  - 36766-9
AB  - Dendritic cells (DCs) play a critical role in the initiation of the immunological 
      response against Leishmania parasites. However, the receptors involved in 
      amastigote-dendritic cell interaction are unknown, especially in absence of 
      opsonizing antibodies. We have studied the interaction of Leishmania pifanoi 
      axenic amastigotes with the C-type lectin DC-specific intercellular adhesion 
      molecule (ICAM)-3-grabbing nonintegrin (DC-SIGN, CD209), a receptor for ICAM-2, 
      ICAM-3, human immunodeficiency virus gp120, and Ebola virus. L. pifanoi 
      amastigotes interact with immature human dendritic cells and CD209-transfected 
      K562 cells in a time- and dose-dependent manner. Leishmania amastigote binding to 
      human dendritic cells and DC-SIGN-transfected cells is inhibited by a 
      function-blocking DC-SIGN-specific monoclonal antibody. More importantly, this 
      monoclonal antibody dramatically reduces internalization of Leishmania 
      amastigotes by immature human DCs. These results constitute the first description 
      of a nonviral pathogen ligand for DC-SIGN and provide evidence for a relevant 
      role of DC-SIGN in Leishmania amastigote uptake by dendritic cells. Our finding 
      has important implications for Leishmania host-cell interaction and the 
      immunoregulation of cutaneous leishmaniasis.
FAU - Colmenares, Maria
AU  - Colmenares M
AD  - Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones 
      Cientificas, Velazquez 144, 28006 Madrid, Spain.
FAU - Puig-Kroger, Amaya
AU  - Puig-Kroger A
FAU - Pello, Oscar Muniz
AU  - Pello OM
FAU - Corbi, Angel L
AU  - Corbi AL
FAU - Rivas, Luis
AU  - Rivas L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020716
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (ICAM3 protein, human)
RN  - 0 (Lectins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Antigens, CD/metabolism/*physiology
MH  - Antigens, Differentiation/metabolism/*physiology
MH  - Cell Adhesion Molecules/*metabolism
MH  - Dendritic Cells/metabolism/parasitology
MH  - Dose-Response Relationship, Drug
MH  - Ebolavirus/metabolism
MH  - Flow Cytometry
MH  - HIV Envelope Protein gp120/*metabolism
MH  - Humans
MH  - K562 Cells/*parasitology
MH  - Lectins/*metabolism
MH  - Lectins, C-Type/*metabolism
MH  - Leishmania/*metabolism
MH  - Protein Binding
MH  - Protozoan Proteins/*metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Time Factors
EDAT- 2002/07/18 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/07/18 10:00
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - S0021-9258(18)36658-4 [pii]
AID - 10.1074/jbc.M205270200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Sep 27;277(39):36766-9. doi: 10.1074/jbc.M205270200. Epub 2002 
      Jul 16.