PMID- 12122447
OWN - NLM
STAT- MEDLINE
DCOM- 20030715
LR  - 20161124
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 118
IP  - 1
DP  - 2002 Jul
TI  - Expression of neurotrophins and their receptors in peripheral lung cells of mice.
PG  - 51-8
AB  - Neurotrophins play an essential role in nerve systems. Recent reports indicated 
      that neurotrophins [nerve growth factor (NGF), brain-derived neurotrophic factor 
      (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4/5)] have numerous 
      effects on non-neural cells, especially on immune cells. However, whether lung 
      cells express neurotrophins and/or their receptors (TrkA for NGF, TrkB for BDNF 
      and NT-4/5, and TrkC for NT-3) has never been systematically investigated. We 
      investigated constitutive expression of neurotrophin family and their Trk 
      receptor family in alveolar macrophages and other peripheral lung cells of mice. 
      New findings were: (1) RT-PCR for neurotrophins and their receptors detected NT-3 
      and NT-4/5 in alveolar macrophages, BDNF, NT-4/5, trkA, the truncated form of 
      trkB, and trkC in lung homogenate, but no trks in alveolar macrophages, (2) 
      immunohistochemistry for neurotrophin receptors detected TrkA in capillary cells, 
      the truncated form of TrkB, and TrkC in interstitial macrophages, (3) 
      immunoelectron microscopy for TrkC revealed expression of TrkC on the surface of 
      interstitial macrophages, and (4) in situ hybridization for neurotrophins 
      detected BDNF in interstitial macrophages and alveolar type I cells, NT-3 in 
      alveolar macrophages, and NT-4/5 in alveolar and interstitial macrophages. These 
      findings indicate that a previously unknown signal trafficking occurs through 
      neurotrophins in peripheral lung.
FAU - Hikawa, Shiori
AU  - Hikawa S
AD  - Department of Medicine, Kitasato University School of Medicine, Kitasato 1-15-1, 
      Sagamihara, Kanagawa 228-8555, Japan.
FAU - Kobayashi, Hirosuke
AU  - Kobayashi H
FAU - Hikawa, Naoshi
AU  - Hikawa N
FAU - Kusakabe, Tatsumi
AU  - Kusakabe T
FAU - Hiruma, Hiromi
AU  - Hiruma H
FAU - Takenaka, Toshifumi
AU  - Takenaka T
FAU - Tomita, Tomoyuki
AU  - Tomita T
FAU - Kawakami, Tadashi
AU  - Kawakami T
LA  - eng
PT  - Journal Article
DEP - 20020612
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 145172-44-7 (neurotrophin 5)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics
MH  - Carrier Proteins/analysis/genetics
MH  - Immunohistochemistry
MH  - Lung/*cytology
MH  - Macrophages, Alveolar/*chemistry
MH  - Membrane Proteins/analysis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Growth Factors/analysis/genetics/*metabolism/physiology
MH  - Neurotrophin 3/analysis/genetics
MH  - Protein Transport
MH  - RNA, Messenger/analysis
MH  - *Receptor, trkA
MH  - Receptor, trkB/analysis/genetics
MH  - Receptor, trkC/analysis/genetics
MH  - Receptors, Nerve Growth Factor/genetics/*metabolism/physiology
EDAT- 2002/07/18 10:00
MHDA- 2003/07/16 05:00
CRDT- 2002/07/18 10:00
PHST- 2002/05/17 00:00 [accepted]
PHST- 2002/07/18 10:00 [pubmed]
PHST- 2003/07/16 05:00 [medline]
PHST- 2002/07/18 10:00 [entrez]
AID - 10.1007/s00418-002-0426-y [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2002 Jul;118(1):51-8. doi: 10.1007/s00418-002-0426-y. Epub 
      2002 Jun 12.