PMID- 12123476
OWN - NLM
STAT- MEDLINE
DCOM- 20020919
LR  - 20191106
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 4
IP  - 7
DP  - 2002 Jul
TI  - Characterization of the bacterial consortium associated with black band disease 
      in coral using molecular microbiological techniques.
PG  - 401-13
AB  - The bacterial community associated with black band disease (BBD) of the 
      scleractinian corals Diploria strigosa, Montastrea annularis and Colpophyllia 
      natans was examined using culture-independent techniques. Two complementary 
      molecular screening techniques of 16S rDNA genes [amplified 16S ribosomal DNA 
      restriction analysis (ARDRA) of clone libraries and denaturing gradient gel 
      electrophoresis (DGGE)] were used to give a comprehensive characterization of the 
      community. Findings support previous studies indicating low bacterial abundance 
      and diversity associated with healthy corals. A single cyanobacterial ribotype 
      was present in all the diseased samples, but this was not the same as that 
      identified from Phormidium corallyticum culture isolated from BBD. The study 
      confirms the presence of Desulfovibrio spp. and sulphate-reducing bacteria that 
      have previously been associated with the BBD consortium. However, the species 
      varied between diseased coral samples. We found no evidence of bacteria from 
      terrestrial, freshwater or human sources in any of the samples. We report the 
      presence of previously unrecognized potential pathogens [a Cytophaga sp. and an 
      alpha-proteobacterium identified as the aetiological agent of juvenile oyster 
      disease (JOD)] that were consistently present in all the diseased coral samples. 
      The molecular biological approach described here gives an increasingly 
      comprehensive and more precise picture of the bacterial population associated 
      with BBD. To understand the pathogenesis of BBD, our attention should be focused 
      on the pervasive ribotypes identified in this study (the Cyanobacterium sp., the 
      Cytophaga sp. and the JOD pathogen).
FAU - Cooney, Rory P
AU  - Cooney RP
AD  - Department of Marine Sciences and Coastal Management, Ridley Building, University 
      of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK. r.p.cooney@ncl.ac.uk
FAU - Pantos, Olga
AU  - Pantos O
FAU - Le Tissier, Martin D A
AU  - Le Tissier MD
FAU - Barer, Michael R
AU  - Barer MR
FAU - O'Donnell, Anthony G
AU  - O'Donnell AG
FAU - Bythell, John C
AU  - Bythell JC
LA  - eng
SI  - GENBANK/AF473908
SI  - GENBANK/AF473909
SI  - GENBANK/AF473910
SI  - GENBANK/AF473911
SI  - GENBANK/AF473912
SI  - GENBANK/AF473913
SI  - GENBANK/AF473914
SI  - GENBANK/AF473915
SI  - GENBANK/AF473916
SI  - GENBANK/AF473917
SI  - GENBANK/AF473918
SI  - GENBANK/AF473919
SI  - GENBANK/AF473920
SI  - GENBANK/AF473921
SI  - GENBANK/AF473922
SI  - GENBANK/AF473923
SI  - GENBANK/AF473924
SI  - GENBANK/AF473925
SI  - GENBANK/AF473926
SI  - GENBANK/AF473927
SI  - GENBANK/AF473928
SI  - GENBANK/AF473929
SI  - GENBANK/AF473930
SI  - GENBANK/AF473931
SI  - GENBANK/AF473932
SI  - GENBANK/AF473933
SI  - GENBANK/AF473934
SI  - GENBANK/AF473935
SI  - GENBANK/AF473936
SI  - GENBANK/AF473937
SI  - GENBANK/AF473938
SI  - GENBANK/AF473939
SI  - GENBANK/AF473940
SI  - GENBANK/AF473941
SI  - GENBANK/AF473942
SI  - GENBANK/AF473943
SI  - GENBANK/AF473944
SI  - GENBANK/AF473945
SI  - GENBANK/AF473946
SI  - GENBANK/AF473947
SI  - GENBANK/AF473948
SI  - GENBANK/AF473949
SI  - GENBANK/AF473950
SI  - GENBANK/AF473951
SI  - GENBANK/AF473952
SI  - GENBANK/AF473953
SI  - GENBANK/AF473954
SI  - GENBANK/AF473955
SI  - GENBANK/AF473956
SI  - GENBANK/AF473957
SI  - GENBANK/AF473958
SI  - GENBANK/AF473959
SI  - GENBANK/AF473960
SI  - GENBANK/AF473961
SI  - GENBANK/AF473962
SI  - GENBANK/AF473963
SI  - GENBANK/AF473964
SI  - GENBANK/AF473965
SI  - GENBANK/AF473966
SI  - GENBANK/AF473967
SI  - GENBANK/AF473968
SI  - GENBANK/AF473969
SI  - GENBANK/AF473970
SI  - GENBANK/AF473971
SI  - GENBANK/AF473972
SI  - GENBANK/AF473973
SI  - GENBANK/AF473974
SI  - GENBANK/AF473975
SI  - GENBANK/AF473976
SI  - GENBANK/AF473977
SI  - GENBANK/AF473978
SI  - GENBANK/AF473979
SI  - GENBANK/AF473980
SI  - GENBANK/AF473981
SI  - GENBANK/AF473982
SI  - GENBANK/AF473983
SI  - GENBANK/AF473984
SI  - GENBANK/AF473985
SI  - GENBANK/AF473986
SI  - GENBANK/AF473987
SI  - GENBANK/AF473988
SI  - GENBANK/AF473989
SI  - GENBANK/AF473990
SI  - GENBANK/AF473991
SI  - GENBANK/AF473992
SI  - GENBANK/AF473993
SI  - GENBANK/AF473994
SI  - GENBANK/AF473995
SI  - GENBANK/AF473996
SI  - GENBANK/AF473997
SI  - GENBANK/AF473998
SI  - GENBANK/AF473999
SI  - GENBANK/AF474000
SI  - GENBANK/AF474001
SI  - GENBANK/AF474002
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/*pathogenicity
MH  - Bacterial Typing Techniques
MH  - Cnidaria/*microbiology
MH  - DNA Fingerprinting/methods
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - DNA, Ribosomal/analysis
MH  - *Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/microbiology
MH  - Sequence Analysis, DNA
EDAT- 2002/07/19 10:00
MHDA- 2002/09/20 10:01
CRDT- 2002/07/19 10:00
PHST- 2002/07/19 10:00 [pubmed]
PHST- 2002/09/20 10:01 [medline]
PHST- 2002/07/19 10:00 [entrez]
AID - 308 [pii]
AID - 10.1046/j.1462-2920.2002.00308.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2002 Jul;4(7):401-13. doi: 10.1046/j.1462-2920.2002.00308.x.