PMID- 12123556
OWN - NLM
STAT- MEDLINE
DCOM- 20020813
LR  - 20101118
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 115
IP  - 6
DP  - 2002 Jun
TI  - Preimplantation gender diagnosis by fluorescence in situ hybridization.
PG  - 874-7
AB  - OBJECTIVE: To describe the clinical application of fluorescence in situ 
      hybridization (FISH ) in preimplantation gender diagnosis. METHODS: 
      Preimplantation gender diagnosis was performed in 2 female hemophilia A carriers, 
      1 male patient with glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and 2 
      male patients with Y chromosome abnormality. Embryo sex was identified by FISH in 
      total of 6 treatment cycles. RESULTS: A total of 123 cumulus-oocytes were 
      retrieved in 6 treatment cycles. Sixty-one embryos were available for embryo 
      biopsy. The success rate of biopsy was 86.9% (53/61), with a further cleavage 
      rate of 62.3% (33/53). In the FISH procedure, one cell was lost during fixation, 
      leading to a 98.1% (52/53) fixation rate. Totally, 16 female embryos and 3 male 
      embryos were transferred to 5 patients in 6 cycles. Three healthy babies were 
      born. The diagnosis was confirmed by subsequent analysis of amniocytes and 
      embryonic buds after embryo reduction. CONCLUSIONS: FISH is an efficient and 
      reliable technique for determining the sex of human preimplantation embryos. 
      Selective abortion and births of affected children can be avoided by 
      preimplantation gender diagnosis.
FAU - Xu, Yanwen
AU  - Xu Y
AD  - Reproductive Research Center, Department of Obstetrics and Gynecology, The First 
      Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 
      510080, China. xuyanwen@public.guangzhou.gd.cn
FAU - Zhuang, Guanglun
AU  - Zhuang G
FAU - Shu, Yimin
AU  - Shu Y
FAU - Li, Man
AU  - Li M
FAU - Zhou, Canquan
AU  - Zhou C
FAU - Zhang, Minfang
AU  - Zhang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Adult
MH  - Amniocentesis
MH  - Biopsy
MH  - *Blastocyst
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - *Sex Determination Analysis
EDAT- 2002/07/19 10:00
MHDA- 2002/08/14 10:01
CRDT- 2002/07/19 10:00
PHST- 2002/07/19 10:00 [pubmed]
PHST- 2002/08/14 10:01 [medline]
PHST- 2002/07/19 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2002 Jun;115(6):874-7.