PMID- 12124450
OWN - NLM
STAT- MEDLINE
DCOM- 20020829
LR  - 20200218
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 83
IP  - Pt 8
DP  - 2002 Aug
TI  - Identification of radically different variants of porcine reproductive and 
      respiratory syndrome virus in Eastern Europe: towards a common ancestor for 
      European and American viruses.
PG  - 1861-1873
LID - 10.1099/0022-1317-83-8-1861 [doi]
AB  - We determined 22 partial porcine reproductive and respiratory syndrome virus 
      (PRRSV) ORF5 sequences, representing pathogenic field strains mainly from Poland 
      and Lithuania, and two currently available European-type live PRRSV vaccines. 
      Also, the complete ORF7 of two Lithuanian and two Polish strains was sequenced. 
      We found that Polish, and in particular Lithuanian, PRRSV sequences were 
      exceptionally different from the European prototype, the Lelystad virus, and in 
      addition showed a very high national diversity. The most diverse present-day 
      European-type PRRSV sequences were from Poland (2000) and Lithuania (2000), and 
      exhibited only 72.2% nucleotide identity in the investigated ORF5 sequence. While 
      all sequences determined in the present study were clearly of European type, 
      inclusion of the new Lithuanian sequences in the genealogy resulted in a common 
      ancestor for the European type virus significantly closer to the American-type 
      PRRSV than previously seen. In addition, the length of the ORF7 of the Lithuanian 
      strains was 378 nucleotides, and thus intermediate between the sizes of the 
      prototypical EU-type (387 nucleotides) and US-type (372 nucleotides) ORF7 
      lengths. These findings for the Lithuanian PRRSV sequences provide support for 
      the hypothesis that the EU and US genotypes of PRRSV evolved from a common 
      ancestor. Also, this is the first report of ORF7 protein size polymorphism in 
      field isolates of EU-type PRRSV.
FAU - Stadejek, T
AU  - Stadejek T
AD  - National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, 
      Poland1.
FAU - Stankevicius, A
AU  - Stankevicius A
AD  - National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, 
      Poland1.
FAU - Storgaard, T
AU  - Storgaard T
AD  - Danish Veterinary Institute for Virus Research, Lindholm, 4771 Kalvehave, 
      Denmark2.
FAU - Oleksiewicz, M B
AU  - Oleksiewicz MB
AD  - Danish Veterinary Institute for Virus Research, Lindholm, 4771 Kalvehave, 
      Denmark2.
FAU - Belak, S
AU  - Belak S
AD  - National Veterinary Institute, Department of Virology, Biomedical Center, Box 
      585, S-751 23 Uppsala, Sweden3.
FAU - Drew, T W
AU  - Drew TW
AD  - Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey KT15 3NB, UK4.
FAU - Pejsak, Z
AU  - Pejsak Z
AD  - National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, 
      Poland1.
LA  - eng
SI  - GENBANK/AF378800
SI  - GENBANK/AF378801
SI  - GENBANK/AF378802
SI  - GENBANK/AF378803
SI  - GENBANK/AF378804
SI  - GENBANK/AF378805
SI  - GENBANK/AF378806
SI  - GENBANK/AF378807
SI  - GENBANK/AF378808
SI  - GENBANK/AF378809
SI  - GENBANK/AF378810
SI  - GENBANK/AF378811
SI  - GENBANK/AF378812
SI  - GENBANK/AF378813
SI  - GENBANK/AF378814
SI  - GENBANK/AF378815
SI  - GENBANK/AF378816
SI  - GENBANK/AF378817
SI  - GENBANK/AF378818
SI  - GENBANK/AF378819
SI  - GENBANK/AF378820
SI  - GENBANK/AF385293
SI  - GENBANK/AF385294
SI  - GENBANK/AF385295
SI  - GENBANK/AF385296
SI  - GENBANK/AF385297
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Evolution, Molecular
MH  - *Genetic Variation
MH  - Lithuania
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - Phylogeny
MH  - Poland
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/*classification/*genetics
MH  - *Sequence Analysis, DNA
MH  - Swine
MH  - United States
MH  - Viral Envelope Proteins
MH  - Viral Proteins/chemistry/genetics
MH  - Viral Vaccines/genetics
EDAT- 2002/07/19 10:00
MHDA- 2002/08/30 10:01
CRDT- 2002/07/19 10:00
PHST- 2002/07/19 10:00 [pubmed]
PHST- 2002/08/30 10:01 [medline]
PHST- 2002/07/19 10:00 [entrez]
AID - 10.1099/0022-1317-83-8-1861 [doi]
PST - ppublish
SO  - J Gen Virol. 2002 Aug;83(Pt 8):1861-1873. doi: 10.1099/0022-1317-83-8-1861.