PMID- 12125508
OWN - NLM
STAT- MEDLINE
DCOM- 20021218
LR  - 20161020
IS  - 1088-5412 (Print)
IS  - 1088-5412 (Linking)
VI  - 23
IP  - 3
DP  - 2002 May-Jun
TI  - Identification of immunoglobulin E binding components of the two-spotted spider 
      mite Tetranychus urticae: allergenic relationships with the citrus red mite and 
      house-dust mite.
PG  - 199-204
AB  - The two-spotted mite (TSM) is commonly found on fruit trees, herbaceous plants, 
      and greenhouse flowers. However, a recent investigation indicated that the 
      sensitization rate to TSM was as high as that of house-dust mites (HDMs) in 
      nonfarmers as well as in farmers working in orchards in this country. The aim of 
      this study was to identify immunoglobulin E (IgE)-binding components within TSM 
      and to evaluate the allergenic relationship with the citrus red mite (CRM) and 
      HDM. Sera were collected from eight patients who were not farmers and who had 
      asthma with high serum-specific IgE to the TSM and from unexposed controls 
      showing negative responses to the TSM on skin-prick test. Twelve percent sodium 
      dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis were 
      applied. To evaluate allergenic relationships with HDMs and CRMs, two kinds of 
      sera pools were used: one (A) showing positive responses to both TSMs and HDMs 
      and the other (B) showing an isolated positive response to TSMs. ELISA inhibition 
      tests using A and B pooled sera were conducted. The TSM-ELISA inhibition test 
      using sera A showed significant inhibition with addition of TSMs and CRMs, 
      partial inhibition with HDMs, and minimal inhibition with other inhalant 
      allergens. The ELISA inhibition test using sera B showed significant inhibition 
      with TSMs and CRMs and minimal inhibition was noted with HDMs as well as other 
      inhalant allergens. Immunoblot analysis using individual sera showed seven 
      IgE-binding components (75, 56, 47, 41, 37, 28, and 14 kDa) and two (75 and 14 
      kDa) of them were bound to IgE in > 50% of the sera tested. Seven IgE-binding 
      components were identified within the TSM extract and two (75 and 14 kDa) could 
      be considered major allergens. It is suggested that the TSM contains 
      species-specific allergen in addition to shared allergens with CRMs and HDMs.
FAU - Park, Hae-Sim
AU  - Park HS
FAU - Jee, Young-Koo
AU  - Jee YK
FAU - Kim, Youn-Keun
AU  - Kim YK
FAU - Lee, Soo-Keol
AU  - Lee SK
FAU - Lee, Myung-Hyun
AU  - Lee MH
FAU - Kim, You-Young
AU  - Kim YY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Allergy Asthma Proc
JT  - Allergy and asthma proceedings
JID - 9603640
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Asthma/*blood/immunology
MH  - Dermatophagoides farinae/immunology/*pathogenicity
MH  - Dermatophagoides pteronyssinus/immunology/*pathogenicity
MH  - Humans
MH  - Hypersensitivity/*blood/*etiology/immunology
MH  - Immunoblotting
MH  - Immunoglobulin E/*blood/immunology
MH  - Male
MH  - Middle Aged
MH  - Receptors, IgE/*blood/immunology
MH  - Tetranychidae/immunology/*pathogenicity
EDAT- 2002/07/20 10:00
MHDA- 2002/12/19 04:00
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2002/12/19 04:00 [medline]
PHST- 2002/07/20 10:00 [entrez]
PST - ppublish
SO  - Allergy Asthma Proc. 2002 May-Jun;23(3):199-204.