PMID- 12125770
OWN - NLM
STAT- MEDLINE
DCOM- 20030116
LR  - 20190606
IS  - 0305-7364 (Print)
IS  - 1095-8290 (Electronic)
IS  - 0305-7364 (Linking)
VI  - 90
IP  - 1
DP  - 2002 Jul
TI  - Fluorescence in situ hybridization analysis of alien genes in 
      Agrobacterium-mediated Cry1A(b)-transformed rice.
PG  - 31-6
AB  - The transgene in Agrobacterium-mediated Cry1A(b)-transgenic rice plants has been 
      detected and its chromosomal location determined by fluorescence in situ 
      hybridization (FISH). Eight of the nine transgenic lines tested showed 
      hybridization signals. Signals were located on regions of the chromosome in which 
      fraction length (FL) values varied from 26.2 (near the centromere) to 95.2 
      (distal regions). No signal was found on regions where the fraction length was 
      less than 26.2, while six of the nine signals detected were located on regions 
      with FL values of 75.3 or over. This demonstrates that Agrobacterium-mediated 
      genes can integrate into multiple sites distributed in different parts of the 
      chromosome, but that distal regions are the preferred sites and regions near the 
      centromeres are colder for T-DNA integration. The donor DNA of the transformation 
      was divided into two parts, labelled separately as probes for two-colour FISH. 
      Results show that the transformed DNA sequences remained linked in the recipient 
      genome. The relationship between integration position and transgene silencing, 
      known as the 'position effect', is discussed.
FAU - Jin, Wei-Wei
AU  - Jin WW
AD  - Key Lab of MOE for Plant Developmental Biology, Wuhan University, PR China.
FAU - Li, Zong-Yun
AU  - Li ZY
FAU - Fang, Qing
AU  - Fang Q
FAU - Altosaar, Illimar
AU  - Altosaar I
FAU - Liu, Li-Hua
AU  - Liu LH
FAU - Song, Yun-Chun
AU  - Song YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Bot
JT  - Annals of botany
JID - 0372347
RN  - 0 (Cryptochromes)
RN  - 0 (DNA Probes)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Flavoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (cry protein, Drosophila)
SB  - IM
MH  - Cryptochromes
MH  - DNA Probes
MH  - *Drosophila Proteins
MH  - *Eye Proteins
MH  - Flavoproteins/*genetics
MH  - *Genes, Bacterial
MH  - In Situ Hybridization, Fluorescence
MH  - Oryza/*genetics
MH  - *Photoreceptor Cells, Invertebrate
MH  - RNA, Messenger/genetics
MH  - Receptors, G-Protein-Coupled
MH  - *Transformation, Genetic
PMC - PMC4233863
EDAT- 2002/07/20 10:00
MHDA- 2003/01/17 04:00
PMCR- 2003/07/01
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2003/01/17 04:00 [medline]
PHST- 2002/07/20 10:00 [entrez]
PHST- 2003/07/01 00:00 [pmc-release]
AID - 10.1093/aob/mcf160 [doi]
PST - ppublish
SO  - Ann Bot. 2002 Jul;90(1):31-6. doi: 10.1093/aob/mcf160.