PMID- 12126514
OWN - NLM
STAT- MEDLINE
DCOM- 20021122
LR  - 20061115
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 82
IP  - 13
DP  - 2002 Jul
TI  - [The expression of human specific proteins in liver tissue of chimeric goats 
      engrafted with human hematopoitic stem cells].
PG  - 894-8
AB  - OBJECTIVE: To investigate the expression of human specific proteins in liver 
      tissue obtained from goats engrafted with human hematopoietic stem cells (hHSC). 
      METHODS: hHSCs derived from cord blood were transplanted into fetal goats for 
      production of chimerism. Liver specimens were obtained from four 10-month-old 
      chimeric goats and examined for their reactivity with monoclonal antibodies 
      against human antigens: proliferating cell nuclear antigen (PCNA) and hepatocyte 
      specific antigen (HSA). The presence of human HSA positive cells in the goat 
      liver tissue was determined by fluorescence assisted cell sorting (FACS). The 
      expression of human hepatocyte nuclear factor (hHNF-3beta) and human serum 
      albumin (hALB) mRNAs was determined by RT-PCR. Meanwhile, FISH experiment was 
      also performed to detect the human cells in the chimeric livers with the probe of 
      human p17H8. RESULTS: Successful engraftment was confirmed by the detection of 
      human blood cell chimerism in the circulation of the transplanted goats. Human 
      PCNA and HSA were found in liver specimens of transplanted goats but not of 
      normals. FACS analysis showed the presence of human HSA positive cells in the 
      liver of chimeric goats. RT-PCR results demonstrated that hHNF-3beta and hALB 
      mRNAs were specifically expressed in liver tissues of chimeric goats. FISH 
      experiment showed two positive hybridized signals in some liver cells of the 
      chimeric goats, indicating that human liver-like cells were present there. 
      CONCLUSION: Goats engrafted with hHSC are capable to produce chimeric livers. 
      Growth and propagation of human cells in the liver tissue of such transplanted 
      goats were possible. Particularly, the human liver-like cells in chimeric goat 
      livers had transcriptional activity specific to human hepatocytes.
FAU - Huang, Shuzhen
AU  - Huang S
AD  - Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 
      200040, China.
FAU - Yam, Hinfai
AU  - Yam H
FAU - Pang, Chipui
AU  - Pang C
FAU - Chen, Meijue
AU  - Chen M
FAU - Gong, Zhijuan
AU  - Gong Z
FAU - Zeng, Fanyi
AU  - Zeng F
FAU - Ling, Shunchiu
AU  - Ling S
FAU - Zeng, Yitao
AU  - Zeng Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FOXA2 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
RN  - 0 (Transcription Factors)
RN  - 135845-92-0 (Hepatocyte Nuclear Factor 3-beta)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/*genetics
MH  - Goats
MH  - Hepatocyte Nuclear Factor 3-beta
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Liver/*metabolism
MH  - Nuclear Proteins/*genetics
MH  - RNA, Messenger/analysis
MH  - Serum Albumin/*genetics
MH  - *Stem Cell Transplantation
MH  - *Transcription Factors
MH  - Transplantation Chimera/*metabolism
EDAT- 2002/07/20 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/07/20 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2002 Jul;82(13):894-8.