PMID- 12127057
OWN - NLM
STAT- MEDLINE
DCOM- 20030214
LR  - 20190922
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 32
IP  - 1
DP  - 2002 Jul
TI  - AT1 angiotensin II receptor mediates intracellular calcium mobilization in normal 
      and cancerous breast cells in primary culture.
PG  - 1-10
AB  - Angiotensin II (Ang II) increases intracellular calcium concentration ([Ca2+]i) 
      in both normal and cancerous human breast cells in primary culture. Maximal 
      [Ca2+]i increase is obtained using 100nM Ang II in both cell types; in cancerous 
      breast cells, [Ca2+]i increase (delta[Ca2+]i) is 135+/-10nM, while in normal 
      breast cells it reaches 65+/-5 nM (P<0.0001). In both cell types, Ang II evokes a 
      Ca2+ transient peak mediated by thapsigargin (TG) sensitive stores; neither Ca2+ 
      entry through L-type membrane channels or capacitative Ca2+ entry are involved. 
      Type I Ang II receptor subtype (AT1) mediates Ang II-dependent [Ca2+]i increase, 
      since losartan, an AT1 inhibitor, blunted [Ca2+]i increase induced by Ang II in a 
      dose-dependent manner, while CGP 4221A, an AT2 inhibitor, does not. Phospholipase 
      C (PLC) is involved in this signaling mechanism, as U73122, a PLC inhibitor, 
      decreases Ang II-dependent [Ca2+]i transient peak in a dose-dependent mode.Thus, 
      the present study provides new information about Ca2+ signaling pathways mediated 
      through AT1 in breast cells in which no data were yet available.
FAU - Greco, S
AU  - Greco S
AD  - Laboratorio di Fisiologia Generale, Dipartimento di Scienze e Tecnologie 
      Biologiche e Ambientali, Universita di Lecce, Via Provinciale per Monteroni, 
      73100 Lecce, Italy.
FAU - Elia, M G
AU  - Elia MG
FAU - Muscella, A
AU  - Muscella A
FAU - Storelli, C
AU  - Storelli C
FAU - Marsigliante, S
AU  - Marsigliante S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Receptor, Angiotensin, Type 1)
RN  - 0 (Receptors, Angiotensin)
RN  - 0 (Vasoconstrictor Agents)
RN  - 11128-99-7 (Angiotensin II)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Angiotensin II/pharmacology
MH  - *Breast Neoplasms
MH  - Calcium/*metabolism
MH  - Calcium Signaling/drug effects/physiology
MH  - Epithelial Cells/cytology/drug effects/metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Receptor, Angiotensin, Type 1
MH  - Receptors, Angiotensin/genetics/*metabolism
MH  - Tumor Cells, Cultured
MH  - Type C Phospholipases/metabolism
MH  - Vasoconstrictor Agents/pharmacology
EDAT- 2002/07/20 10:00
MHDA- 2003/02/15 04:00
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2003/02/15 04:00 [medline]
PHST- 2002/07/20 10:00 [entrez]
AID - S0143416002000775 [pii]
AID - 10.1016/s0143-4160(02)00077-5 [doi]
PST - ppublish
SO  - Cell Calcium. 2002 Jul;32(1):1-10. doi: 10.1016/s0143-4160(02)00077-5.