PMID- 12127062
OWN - NLM
STAT- MEDLINE
DCOM- 20030214
LR  - 20190922
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 32
IP  - 1
DP  - 2002 Jul
TI  - Xestospongin C empties the ER calcium store but does not inhibit InsP3-induced 
      Ca2+ release in cultured dorsal root ganglia neurones.
PG  - 49-52
AB  - The action of Xestospongin C (XeC) on calcium concentration in the cytosol 
      ([Ca2+]i) and within the lumen of endoplasmic reticulum (ER) ([Ca2+]L) was 
      studied using cultured dorsal root ganglia (DRG) neurones. Application of 2.5 
      microM of XeC triggered a slow [Ca2+]i transient as measured by Fura-2 
      video-imaging. The kinetics and amplitude of XeC-induced [Ca2+]i response was 
      similar to that triggered by 1 microM thapsigargin (TG). The [Ca2+]L was 
      monitored in cells loaded with low-affinity Ca2+ indicator Mag-Fura-2. The 
      cytosolic portion of Mag-Fura-2 was removed by permeabilisation of the 
      plasmalemma with saponin. Application of XeC to these permeabilised neurones 
      resulted in a slow depletion of the ER Ca2+ store. XeC, however, failed to 
      inhibit inositol 1,4,5-trisphosphate (InsP3)-induced [Ca2+]L responses. We 
      conclude that XeC is a potent inhibitor of sarco(endo)plasmic reticulum calcium 
      ATPase, and it cannot be regarded as a specific inhibitor of InsP3 receptors in 
      cultured DRG neurones.
FAU - Solovyova, N
AU  - Solovyova N
AD  - School of Biological Sciences, The University of Manchester, 1.124 Stopford 
      Building, Oxford Road, Manchester, UK.
FAU - Fernyhough, P
AU  - Fernyhough P
FAU - Glazner, G
AU  - Glazner G
FAU - Verkhratsky, A
AU  - Verkhratsky A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Macrocyclic Compounds)
RN  - 0 (Oxazoles)
RN  - 0 (xestospongin A)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Endoplasmic Reticulum/*metabolism
MH  - Enzyme Inhibitors/*pharmacology
MH  - Ganglia, Spinal/cytology
MH  - Inositol 1,4,5-Trisphosphate/*metabolism
MH  - Macrocyclic Compounds
MH  - Neurons, Afferent/cytology/drug effects/*metabolism
MH  - Oxazoles/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/07/20 10:00
MHDA- 2003/02/15 04:00
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2003/02/15 04:00 [medline]
PHST- 2002/07/20 10:00 [entrez]
AID - S0143416002000945 [pii]
AID - 10.1016/s0143-4160(02)00094-5 [doi]
PST - ppublish
SO  - Cell Calcium. 2002 Jul;32(1):49-52. doi: 10.1016/s0143-4160(02)00094-5.