PMID- 12127073
OWN - NLM
STAT- MEDLINE
DCOM- 20020911
LR  - 20190629
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 404
IP  - 1
DP  - 2002 Aug 1
TI  - Cell density inversely regulates D- and L-aspartate levels in rat 
      pheochromocytoma MPT1 cells.
PG  - 92-7
AB  - In a previous report (FEBS Lett. 434 (1998) 231), we demonstrated for the first 
      time that D-aspartate (D-Asp) is synthesized in rat pheochromocytoma 12 (PC12) 
      cells. This unique amino acid is believed to act as a novel messenger in 
      mammalian cell regulation. However, the dynamics of D-Asp homeostasis in 
      mammalian cells is yet to be elucidated. In this communication, we demonstrate 
      that D-Asp is also synthesized in MPT1 cells (a subclone of PC12 cells) and that 
      the D- and L-Asp levels in cells are regulated by cell density of the culture. 
      Our data show that D-Asp levels increase, while in contrast, L-Asp levels 
      decrease as a function of increased cell density. Conversely, in PC12 cells, 
      which do not express the glutamate transporter involved in the incorporation of 
      D- and L-Asp into cells, L-Asp levels decrease upon cell density increase while 
      D-Asp concentrations remain almost unchanged. The results indicate that the 
      biochemical behaviors of D- and L-Asp in mammalian cells are distinct and that 
      the cellular levels of these stereoisomers appear to be under different control 
      mechanisms.
FAU - Long, Zhiqun
AU  - Long Z
AD  - School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
FAU - Sekine, Masae
AU  - Sekine M
FAU - Adachi, Minako
AU  - Adachi M
FAU - Furuchi, Takemitsu
AU  - Furuchi T
FAU - Imai, Kazuhiro
AU  - Imai K
FAU - Nimura, Noriyuki
AU  - Nimura N
FAU - Homma, Hiroshi
AU  - Homma H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 30KYC7MIAI (Aspartic Acid)
SB  - IM
MH  - Animals
MH  - Aspartic Acid/biosynthesis/chemistry/*metabolism
MH  - Cell Count
MH  - Homeostasis
MH  - PC12 Cells
MH  - Pheochromocytoma/*metabolism/pathology
MH  - Rats
MH  - Stereoisomerism
EDAT- 2002/07/20 10:00
MHDA- 2002/09/12 10:01
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2002/09/12 10:01 [medline]
PHST- 2002/07/20 10:00 [entrez]
AID - S0003986102002412 [pii]
AID - 10.1016/s0003-9861(02)00241-2 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2002 Aug 1;404(1):92-7. doi: 10.1016/s0003-9861(02)00241-2.