PMID- 12130492
OWN - NLM
STAT- MEDLINE
DCOM- 20020906
LR  - 20211103
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 100
IP  - 3
DP  - 2002 Aug 1
TI  - Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show 
      increased stability in sera and augmented transduction of primary lymphocytes and 
      CD34+ cells derived from human and nonhuman primates.
PG  - 823-32
AB  - Generating lentiviral vectors pseudotyped with different viral glycoproteins 
      (GPs) may modulate the physicochemical properties of the vectors, their 
      interaction with the host immune system, and their host range. We have 
      investigated the capacity of a panel of GPs of both retroviral (amphotropic 
      murine leukemia virus [MLV-A]; gibbon ape leukemia virus [GALV]; RD114, feline 
      endogenous virus) and nonretroviral (fowl plague virus [FPV]; Ebola virus [EboV]; 
      vesicular stomatitis virus [VSV]; lymphocytic choriomeningitis virus [LCMV]) 
      origins to pseudotype lentiviral vectors derived from simian immunodeficiency 
      virus (SIVmac251). SIV vectors were efficiently pseudotyped with the FPV 
      hemagglutinin, VSV-G, LCMV, and MLV-A GPs. In contrast, the GALV and RD114 GPs 
      conferred much lower infectivity to the vectors. Capitalizing on the conservation 
      of some structural features in the transmembrane domains and cytoplasmic tails of 
      the incorporation-competent MLV-A GP and in RD114 and GALV GPs, we generated 
      chimeric GPs encoding the extracellular and transmembrane domains of GALV or 
      RD114 GPs fused to the cytoplasmic tail (designated TR) of MLV-A GP. Importantly, 
      SIV-derived vectors pseudotyped with these GALV/TR and RD114/TR GP chimeras had 
      significantly higher titers than vectors coated with the parental GPs. 
      Additionally, RD114/TR-pseudotyped vectors were efficiently concentrated and were 
      resistant to inactivation induced by the complement of both human and macaque 
      sera, indicating that modified RD114 GP-pseudotyped lentiviral vectors may be of 
      particular interest for in vivo gene transfer applications. Furthermore, as 
      compared to vectors pseudotyped with other retroviral GPs or with VSV-G, 
      RD114/TR-pseudotyped vectors showed augmented transduction of human and macaque 
      primary blood lymphocytes and CD34+ cells.
FAU - Sandrin, Virginie
AU  - Sandrin V
AD  - Vectorologie Retrovirale & Therapie Genique, U412 INSERM, IFR 74, Ecole Normale 
      Superieure de Lyon, Lyon, France.
FAU - Boson, Bertrand
AU  - Boson B
FAU - Salmon, Patrick
AU  - Salmon P
FAU - Gay, Wilfried
AU  - Gay W
FAU - Negre, Didier
AU  - Negre D
FAU - Le Grand, Roger
AU  - Le Grand R
FAU - Trono, Didier
AU  - Trono D
FAU - Cosset, Francois-Loic
AU  - Cosset FL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD34)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/*blood
MH  - Blood Cells/*metabolism
MH  - Drug Stability
MH  - Genetic Vectors/*genetics/standards
MH  - Hematopoietic Stem Cells/metabolism
MH  - Humans
MH  - Lentivirus/*genetics
MH  - Lymphocytes/immunology/metabolism
MH  - Macaca
MH  - Simian Immunodeficiency Virus/*genetics
MH  - Transduction, Genetic/*methods
MH  - Viral Envelope Proteins/*genetics
EDAT- 2002/07/20 10:00
MHDA- 2002/09/07 10:01
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2002/09/07 10:01 [medline]
PHST- 2002/07/20 10:00 [entrez]
AID - S0006-4971(20)59380-4 [pii]
AID - 10.1182/blood-2001-11-0042 [doi]
PST - ppublish
SO  - Blood. 2002 Aug 1;100(3):823-32. doi: 10.1182/blood-2001-11-0042.