PMID- 12131768
OWN - NLM
STAT- MEDLINE
DCOM- 20030110
LR  - 20190906
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 25
IP  - 1
DP  - 2002 Jul
TI  - Characterization of the neurotrophic response to acute pancreatitis.
PG  - 31-8
AB  - INTRODUCTION: Interesting preliminary data on changes in the neurotrophin system 
      in various digestive diseases have recently begun to emerge. AIMS: To measure 
      changes in messenger RNA (mRNA) levels of neurotrophins and to identify cell 
      types expressing neurotrophins in the pancreas of rats with L-arginine-induced 
      pancreatitis. METHODOLOGY: Rats were killed at time points from 2 hours to 4 
      weeks after the induction of pancreatitis, and responses were measured by assay. 
      RESULTS: By RNase protection assay, ciliary neurotrophic factor (CNTF) mRNA 
      expression showed a rapid response (sixfold increase over control) in the 
      inflamed pancreas at 2 hours. The levels of mRNA expression of brain-derived 
      neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), 
      neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) in the inflamed pancreas reached 
      a peak at 1 week (2.5-fold, twofold, fourfold, and fivefold increase, 
      respectively). By immunohistochemistry, immunoreactivity for all neurotrophins 
      examined was observed in the islets of Langerhans in the control pancreas at all 
      time points, but it was markedly reduced in the islets in the inflamed pancreas 
      at 2 and 6 hours. Acinar and ductal cells, inflammatory cells, and neural 
      elements were immunoreactive for those neurotrophins in the inflamed pancreas 
      from 2 hours to 2 weeks. CONCLUSION: The temporal and spatial expression of 
      neurotrophins in the course of experimental pancreatitis suggests that their 
      upregulation is a critical component of the response of the pancreas to injury in 
      this model.
FAU - Toma, Hiroki
AU  - Toma H
AD  - Enteric Neuromuscular Disorders and Pain Laboratory, Department of Internal 
      Medicine, Division of Gastroenterology and Hepatology, University of Texas 
      Medical Branch, Galveston, Texas 77555, USA.
FAU - Winston, John H
AU  - Winston JH
FAU - Micci, Maria-Adelaide
AU  - Micci MA
FAU - Li, Hui
AU  - Li H
FAU - Hellmich, Helen Lee
AU  - Hellmich HL
FAU - Pasricha, Pankaj J
AU  - Pasricha PJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 94ZLA3W45F (Arginine)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Arginine
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Gene Expression
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Immunohistochemistry
MH  - Islets of Langerhans/innervation/physiopathology
MH  - Male
MH  - Nerve Growth Factors/*genetics
MH  - Neurotrophin 3/genetics
MH  - Pancreatitis/chemically induced/*physiopathology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/07/20 10:00
MHDA- 2003/01/11 04:00
CRDT- 2002/07/20 10:00
PHST- 2002/07/20 10:00 [pubmed]
PHST- 2003/01/11 04:00 [medline]
PHST- 2002/07/20 10:00 [entrez]
AID - 00006676-200207000-00009 [pii]
AID - 10.1097/00006676-200207000-00009 [doi]
PST - ppublish
SO  - Pancreas. 2002 Jul;25(1):31-8. doi: 10.1097/00006676-200207000-00009.