PMID- 12132673
OWN - NLM
STAT- MEDLINE
DCOM- 20030108
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 25
IP  - 7
DP  - 2002 Jul
TI  - Effect of SCG, 1,3-beta-D-glucan from Sparassis crispa on the hematopoietic 
      response in cyclophosphamide induced leukopenic mice.
PG  - 931-9
AB  - Sparassis crispa Fr. is an edible mushroom recently cultivable in Japan. It 
      contains a remarkably high content of 6-branched 1,3-beta-D-glucan showing 
      antitumor activity. Using ion-exchange chromatography, a purified beta-glucan 
      preparation, SCG, was prepared. In this study, we examined the hematopoietic 
      response by SCG in cyclophosphamide (CY)-induced leukopenic mice. SCG enhanced 
      the hematopoietic response in CY induced leukopenic mice by intraperitoneal 
      routes over a wide range of concentrations. SCG enhanced the hematopoietic 
      response in CY-treated mice by prior or post administration. Analyzing the 
      leukocyte population by flow cytometry, monocytes and granulocytes in the 
      peritoneal cavity, liver, spleen and bone marrow (BM) recovered faster than in 
      the control group. The ratio of natural killer cells and gammadelta T cells in 
      the liver, spleen and peritoneal cavity was also increased. In contrast, CD4+ 
      CD8+ cells in the thymus were temporarily significantly decreased by the 
      administration of SCG. Interleukin-6 (IL-6) production of CY+SCG-treated 
      peritoneal exdated cells (PECs), spleen cells and bone marrow cells (BMCs) were 
      higher than that of the CY-treated group. By in vitro culture of CY-treated PEC 
      and spleen cells, IL-6 production was enhanced by the addition of SCG. These 
      facts suggested the possibility that IL-6 might be a key cytokine for the 
      enhanced hematopoietic response by SCG.
FAU - Harada, Toshie
AU  - Harada T
AD  - Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, 
      Tokyo University of Pharmacy & Life Science, Hachioji, Japan.
FAU - Miura, Noriko
AU  - Miura N
FAU - Adachi, Yoshiyuki
AU  - Adachi Y
FAU - Nakajima, Mitsuhiro
AU  - Nakajima M
FAU - Yadomae, Toshiro
AU  - Yadomae T
FAU - Ohn, Naohito
AU  - Ohn N
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Glucans)
RN  - 0 (Interleukin-6)
RN  - 0 (beta-Glucans)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - 9051-97-2 (beta-1,3-glucan)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Cyclophosphamide
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Glucans/administration & dosage/isolation & 
      purification/*pharmacology/therapeutic use
MH  - Injections, Intraperitoneal
MH  - Interleukin-6/biosynthesis
MH  - Leukocytes/cytology/*drug effects/metabolism
MH  - Leukopenia/chemically induced/*drug therapy
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Organ Specificity
MH  - Polyporales/*chemistry
MH  - *beta-Glucans
EDAT- 2002/07/23 10:00
MHDA- 2003/01/09 04:00
CRDT- 2002/07/23 10:00
PHST- 2002/07/23 10:00 [pubmed]
PHST- 2003/01/09 04:00 [medline]
PHST- 2002/07/23 10:00 [entrez]
AID - 10.1248/bpb.25.931 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2002 Jul;25(7):931-9. doi: 10.1248/bpb.25.931.