PMID- 12133311
OWN - NLM
STAT- MEDLINE
DCOM- 20020808
LR  - 20131121
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 115
IP  - 4
DP  - 2002 Apr
TI  - Effect of polysaccharide sulfate 916 on the production of nitric oxide in ECV304 
      cells induced by cytokines and H(2)O(2).
PG  - 614-6
AB  - OBJECTIVE: To investigate the effect of polysaccharide sulfate 916 (PS916) on the 
      production of nitric oxide (NO) in ECV304 cells induced by tumor necrosis 
      factor-alpha (TNF alpha), interleukin-1 beta (IL-1 beta) and H(2)O(2) in vitro. 
      METHODS: Production of NO in ECV304 cells was measured by the Griess method and 
      the proliferation of cells was tested by the MTT method. The activity of NO 
      synthase was detected spectrophotometrically. RESULTS: Production of NO in ECV304 
      cells decreased after treatment with 40 ng/ml IL-1 beta and 40 ng/ml TNF alpha, 
      but increased in the presence of H(2)O(2) 0.1 mmol/L. PS916 significantly 
      enhanced NO production in ECV304 cells in a dose-dependent manner in the TNF 
      alpha and IL-1 beta treated groups and decreased it in the H(2)O(2) treated 
      group. Proliferation of ECV304 cells was inhibited by TNFalpha and H(2)O(2) and 
      no effect was found in the IL-1 beta treated group. PS916 increased the 
      proliferation of cells treated with TNFalpha and H(2)O(2) dose-dependently. In 
      vitro, PS916 has no effect on the activity of NO synthase. CONCLUSION: PS916 has 
      a protective effect on ECV304 cells exposed to IL-1 beta, TNF alpha and H(2)O(2).
FAU - Ren, Decheng
AU  - Ren D
AD  - Institute of Material Medica, Chinese Academy of Medical Sciences, Beijing 
      100050, China. dugh@imm.ac.cn
FAU - Geng, Meiyu
AU  - Geng M
FAU - Du, Guanhua
AU  - Du G
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Polysaccharides)
RN  - 0 (Sulfuric Acids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cytokines/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/cytology/*drug effects/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/*pharmacology
MH  - Interleukin-1/pharmacology
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase/drug effects/metabolism
MH  - Polysaccharides/metabolism/*pharmacology
MH  - Sulfuric Acids/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2002/07/23 10:00
MHDA- 2002/08/09 10:01
CRDT- 2002/07/23 10:00
PHST- 2002/07/23 10:00 [pubmed]
PHST- 2002/08/09 10:01 [medline]
PHST- 2002/07/23 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2002 Apr;115(4):614-6.