PMID- 12133483
OWN - NLM
STAT- MEDLINE
DCOM- 20020909
LR  - 20061115
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 82
IP  - 9
DP  - 2002 May 10
TI  - [The UCSNP44 variation of calpain 10 gene on NIDDM1 locus and its impact on 
      plasma glucose levels in type 2 diabetic patients].
PG  - 613-6
AB  - OBJECTIVE: To study the contribution of single nucleotide polymorphism-UCSNP44 at 
      calpain-10 gene (CAPN-10) on NIDDM1 locus to type 2 diabetes mellitus (T2DM) in 
      Chinese. METHODS: 276 Chinese living in Shanghai, 148 with normal glucose 
      tolerance (NGT) and 128 with T2DM were given 75 g glucose. O, 30, 60, 120, and 
      180 minutes later their plasma glucose (PG), insulin (INS), C-peptide (CP), and 
      free fatty acids (FFA) were measured and the areas under curve (AUC) were 
      calculated. The islet beta-cell insulin secretion and tissue insulin sensitivity 
      were estimated by formulae of homeostasis model assessment and increment ratio of 
      insulin to glucose levels 30 minutes after glucose challenge. The CAPN-10 UCSNP44 
      as well as UCSNP43 were genotyped by automated DNA direct sequencing. RESULTS: 
      (1) The major genotype of CAPN-10 UCSNP44 in persons with NGT was TT (with a 
      frequency of 0.82); the major allele was T (0.91). The most frequent genotype 
      combination between UCSNP44 and UCSNP43 was TT-GG (corresponding to haplotype 
      combination TG/TG) (0.64). The most frequent haplotype was TG (0.80). The D value 
      for linkage disequilibrium between UCSNP44 and UCSNP43 was -0.11. (2) The 
      frequencies of UCSNP44 and UCSNP44/UCSNP43 haplotype combination did not differ 
      significantly between subjects with NGT and those with T2DM. (3) The PG levels in 
      T2DM subjects with UCSNP44 TT genotype both at fasting and after glucose 
      challenge were statistically significantly higher than those in subjects with 
      non-TT (TC + CC) genotype, especially the PG levels 0, 60, 120, and 180 minutes 
      after glucose challenge (P = 0.036, 0.040, 0.020, and 0.017) and the PG-AUC (P = 
      0.013). The PG levels and PG-AUC 0 and 120 minutes after glucose challenge were 
      still significant after adjusted with age, sex, and body mass index and waist 
      circumference. Similar results were observed in comparison between the TG/TG and 
      TG/CG subgroups of UCSNP44/UCSNP43 haplotype combination. In addition, T2DM 
      subjects with UCSNP44 TT genotype had lower CP levels after glucose challenge 
      than those with non-TT genotype. However, the difference became not statistically 
      significant after adjusted with above-mentioned variables. CONCLUSION: The 
      variation of CAPN-10 UCSNP44 has an impact on plasma glucose levels at fasting 
      and after glucose challenge in subjects with type 2 diabetes. The relevant 
      mechanism remains to be elucidated.
FAU - Wang, Yanqing
AU  - Wang Y
AD  - Shanghai Diabetes Institute, Department of Endocrinology & Metabolism, Shanghai 
      No. 6 People Hospital, Shanghai 200233, China.
FAU - Xiang, Kunsan
AU  - Xiang K
FAU - Zheng, Taishan
AU  - Zheng T
FAU - Jia, Weiping
AU  - Jia W
FAU - Shen, Kunxue
AU  - Shen K
FAU - Li, Jie
AU  - Li J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Blood Glucose)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.4.22.- (calpain 10)
SB  - IM
MH  - Blood Glucose/*analysis
MH  - Calpain/*genetics/physiology
MH  - Diabetes Mellitus, Type 2/blood/*genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
EDAT- 2002/07/23 10:00
MHDA- 2002/09/11 10:01
CRDT- 2002/07/23 10:00
PHST- 2002/07/23 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/07/23 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2002 May 10;82(9):613-6.