PMID- 12133594
OWN - NLM
STAT- MEDLINE
DCOM- 20021028
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 946
IP  - 1
DP  - 2002 Aug 9
TI  - A comparative immunocytochemical study of development and regeneration of 
      chemosensory neurons in the rat vomeronasal system.
PG  - 52-63
AB  - Vomeronasal neurons undergo continuous neurogenesis during development and after 
      neuronal injury. We used immunocytochemical methods to compare different stages 
      of the vomeronasal organ development to those of regeneration following 
      vomeronasal nerve transection. At E15 and at 6 to 10 days after injury, 
      nestin-positive cells were observed throughout the sensory epithelium. We did not 
      find nestin immunoreactivity to be localized to the boundary region of the 
      epithelium. The early appearance and wide distribution of nestin-positive cells 
      suggests that they represent chemosensory precursor cells that develop and 
      migrate vertically in the epithelium. Vomeronasal receptor cells degenerated 6 to 
      8 days after nerve transection, but axon terminals in the accessory olfactory 
      bulb (AOB) continued to show the presence of the chemosensory specific marker 
      (OMP) for up to ten days, a significant finding observed in this study. It is 
      likely that the distance from the site of nerve transection may contribute to 
      differences in the time course of anterograde and retrograde axon degradation. 
      OMP-positive neurons were observed in the normal adult epithelium and to a much 
      lesser extent 10-60 days after recovery from nerve transection. Axons from 
      regenerated receptor cells did not reach the AOB during this time period. This 
      failure to reestablish connections with target cells in the AOB could explain why 
      OMP-positive cells were rarely observed among the regenerated cells in the 
      vomeronasal epithelium.
FAU - Matsuoka, Masato
AU  - Matsuoka M
AD  - Division of Neurobiology and Anatomy, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata, Niigata 951-8510, Japan. 
      winner@med.niigata-u.ac.jp
FAU - Osada, Toshiya
AU  - Osada T
FAU - Yoshida-Matsuoka, Junko
AU  - Yoshida-Matsuoka J
FAU - Ikai, Atsushi
AU  - Ikai A
FAU - Ichikawa, Masumi
AU  - Ichikawa M
FAU - Norita, Masao
AU  - Norita M
FAU - Costanzo, Richard M
AU  - Costanzo RM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nes protein, rat)
RN  - 0 (Nestin)
RN  - 0 (Olfactory Marker Protein)
RN  - 0 (Omp protein, rat)
SB  - IM
MH  - Aging/physiology
MH  - Animals
MH  - Chemoreceptor Cells/*physiology
MH  - Denervation
MH  - Embryo, Mammalian/physiology
MH  - Embryonic and Fetal Development
MH  - Immunohistochemistry
MH  - Intermediate Filament Proteins/metabolism
MH  - Nerve Degeneration/metabolism/pathology
MH  - Nerve Endings/embryology/physiology
MH  - Nerve Regeneration/*physiology
MH  - Nerve Tissue Proteins/metabolism
MH  - Nestin
MH  - Olfactory Bulb/embryology/physiology
MH  - Olfactory Marker Protein
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Vomeronasal Organ/*embryology/physiology
EDAT- 2002/07/23 10:00
MHDA- 2002/10/29 04:00
CRDT- 2002/07/23 10:00
PHST- 2002/07/23 10:00 [pubmed]
PHST- 2002/10/29 04:00 [medline]
PHST- 2002/07/23 10:00 [entrez]
AID - S0006899302028238 [pii]
AID - 10.1016/s0006-8993(02)02823-8 [doi]
PST - ppublish
SO  - Brain Res. 2002 Aug 9;946(1):52-63. doi: 10.1016/s0006-8993(02)02823-8.