PMID- 12135683
OWN - NLM
STAT- MEDLINE
DCOM- 20020829
LR  - 20191025
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 30
IP  - 7
DP  - 2002 Jul
TI  - Ischemic cerebral tissue and MCP-1 enhance rat bone marrow stromal cell migration 
      in interface culture.
PG  - 831-6
AB  - OBJECTIVE: Intravascular administration of bone marrow stromal cells (MSCs) 
      restores function in animal models of neural injury and neurodegeneration. Adult 
      MSCs administered intravenously to rat migrate and express neural phenotypes in 
      ischemic brain. The aim of the present study was to investigate the mechanisms 
      targeting MSC migration into the ischemic brain. METHODS: Monocyte 
      chemoattractant protein-1 (MCP-1), a chemoattractant factor, was measured in rat 
      ischemic brain at various time points after middle cerebral artery occlusion 
      (MCAo) using a specific enzyme-linked immunosorbent assay system (ELISA). In 
      addition, using a microchemotaxis chamber, we measured whether ischemic brain 
      tissue extracts induce migration of MSCs and whether brain tissue extracts 
      incubated with antibodies against MCP-1 reduce MSC migration. RESULT: Our data 
      indicate that ischemic brain MCP-1 levels significantly increase from 6 hours, 
      peak at 48 hours after MCAo (p < 0.05), and thereafter gradually decrease. Brain 
      tissue extract at 6 hours, 24 hours, and 48 hours after MCAo significantly 
      increase MSC migration across the membrane of the microchemotaxis chamber 
      compared to normal tissue (p < 0.05). However, when the ischemic brain tissue 
      extracts are incubated with antibody against MCP-1, MSC migration is 
      significantly reduced at 24 hours and 48 hours after MCAo compared to extracts 
      without this antibody (p < 0.05). CONCLUSION: Our data suggest that MCP-1 
      contributes to MSC migration into the ischemic brain tissue environment.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Neurology, Division of Hematology and Medical Oncology, Henry Ford 
      Health Sciences Center, Detroit, MI 48202, USA.
FAU - Li, Yi
AU  - Li Y
FAU - Chen, Jieli
AU  - Chen J
FAU - Gautam, Subhash C
AU  - Gautam SC
FAU - Zhang, Zhenggang
AU  - Zhang Z
FAU - Lu, Mei
AU  - Lu M
FAU - Chopp, Michael
AU  - Chopp M
LA  - eng
GR  - P0I NS23393/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Ccr2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tissue Extracts)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Bone Marrow Cells/drug effects
MH  - *Bone Marrow Transplantation
MH  - Brain Chemistry
MH  - Brain Ischemia/metabolism/*pathology/therapy
MH  - Cell Movement
MH  - Cells, Cultured/drug effects
MH  - Chemokine CCL2/analysis/antagonists & inhibitors/genetics/*physiology
MH  - Chemotaxis/*drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Infarction, Middle Cerebral Artery/metabolism/*pathology/therapy
MH  - Models, Animal
MH  - Nerve Tissue Proteins/analysis/*physiology
MH  - Rats
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/drug effects
MH  - Recombinant Proteins/pharmacology
MH  - Stromal Cells/drug effects/*transplantation
MH  - Tissue Extracts/pharmacology
EDAT- 2002/07/24 10:00
MHDA- 2002/08/30 10:01
CRDT- 2002/07/24 10:00
PHST- 2002/07/24 10:00 [pubmed]
PHST- 2002/08/30 10:01 [medline]
PHST- 2002/07/24 10:00 [entrez]
AID - S0301472X02008299 [pii]
AID - 10.1016/s0301-472x(02)00829-9 [doi]
PST - ppublish
SO  - Exp Hematol. 2002 Jul;30(7):831-6. doi: 10.1016/s0301-472x(02)00829-9.